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M. H. EHLERS and JORGE CHIRIBOGA

Summary.

A technique which depends on absorbing 14CO2 on paper strips containing small amounts of KOH, and 'counting' the strips directly in a liquid-scintillation system, has been applied to studies on spermatozoal metabolism. A small quantity of uniformly-labelled [14C]-glucose or [14C]-fructose has been added to washed spermatozoal suspensions which already contained non-labelled glucose or fructose and the 14CO2 production assessed at the end of a 1 hr incubation period in an air atmosphere, at 38° C. Boar spermatozoa, like those of ram, bull and man, have been shown to utilize glucose preferentially if presented with a mixture of these two sugars.

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D. L. GARNER and M. H. EHLERS

Summary.

Separation of ovine seminal plasma proteins in the disc electrophoretic system revealed a consistent pattern of sixteen fractions with some variation among six rams in the relative amount of minor components, whereas bovine seminal plasma had twelve fractions. In both species, the major seminal plasma components had electrophoretic mobilities somewhat less than did the albumin band of the corresponding blood plasma. The prealbumin region of the seminal plasma electrophoretograms contained four fractions in the ovine samples and three fractions in the bovine samples. Storage of diluted ovine and bovine semen at 5° C did not alter the total protein concentration in the seminal plasma. Standard disc electrophoretic patterns showed that two of the sixteen ovine seminal plasma protein fractions disappeared during such storage. No changes were evident in either the standard or basic electrophoretograms of bovine seminal plasma during storage of semen. Standard disc electrophoretograms of ovine and bovine sperm extracts showed many lightly staining bands, but no progressive changes were evident in these electrophoretic patterns during storage of semen.