Search Results

You are looking at 1 - 5 of 5 items for

  • Author: M. J. A. HARVEY x
Clear All Modify Search
Free access

M. J. A. HARVEY

In the domestic pig, Sus scrofa, sexual dimorphism, as indicated by the presence of the sex chromatin body, is detectable in neurones of the brain and spinal cord (Cantwell, Johnston & Zeller, 1958; Hay & Moore, 1961; Harvey, 1969). Attempts to detect this feature in non-nervous tissue have, with one exception, failed. Hoshino & Toryu (1958) claimed to be able to demonstrate sexual dimorphism in eleven such tissues but these findings have not been substantiated (Hay & Moore, 1961; Harvey, 1969).

During a study on sex chromatin in various tissues including nervous tissue, liver and skin (Harvey, 1969), it was decided to examine foetal tissues including amnion. It was considered that this latter tissue might be suitable for demonstrating sex chromatin, such a feature having been demonstrated in man (Klinger, 1957)

Free access

M. J. A. HARVEY

Summary.

A male pig, suffering from lymphosarcoma, was found to have a genotype 39,XXY/40,XXXY, by the use of peripheral blood culture and sex chromatin studies. This is compared to Klinefelter's syndrome and certain aspects of this condition are briefly discussed, together with the co-existence of the chromosome aberration and the neoplasia.

Free access

D. N. Logue and M. J. A. Harvey

Summary. Bulls heterozygous for the 1/29 Robertsonian translocation showed normal mating behaviour, semen quality and spermatogenesis. The karyotypes of 90 offspring of one heterozygous bull were consistent with a normal 1:1 segregation of the translocation. There was no difference in overall chiasma frequency at diakinesis between heterozygous and normal bulls, and while a trivalent was noted at diakinesis the chiasma position within the No. 1 arm of the trivalent did not appear to be altered when compared with that found in the normal No. 1 bivalent. An increased frequency of aneuploid secondary spermatocytes at the second meiotic division (M II) was found in the heterozygous bulls, the rate of non-disjunction based on the proportion of hypermodal cells being raised from 2·8% in the normal to 6·4% in the heterozygous animals.

Free access

M. Guillomot, K. J. Betteridge, D. Harvey and A. K. Goff

Summary. The uptake of horseradish peroxidase tracer injected into the uterine lumen of the cow was studied during the period of conceptus attachment (Days 18–21; Day 0 = oestrus) and also in cyclic animals. Endocytosis occurred in pregnant and non-pregnant cows but was especially marked when circulating progesterone concentrations were high. By 20 min after injection, the tracer was located in apical endocytotic vesicles and in organelles of the lysosomal system. In addition, some of the horseradish peroxidase-containing vesicles were associated with the lateral membranes of the cells and the tracer was also present in the intercellular spaces and beneath the basal membrane, especially in pregnant animals by the time of conceptus attachment. There was no evidence that pinopod-like functions could be attributed to large cytoplasmic protrusions from endometrial cells. Rather, the protrusions seemed to be involved in secretory processes. The presence of clear vesicles among the endocytotic vesicles suggested a coupled secretory–endocytotic activity of the cells, the significance of which remains to be determined.

Free access

J. P. Renton, J. S. Boyd, P. D. Eckersall, J. M. Ferguson, M. J. A. Harvey, J. Mullaney and B. Perry

Summary. Using circulating plasma hormone estimations, ovulation was monitored in bitches. The results obtained indicate that the timing of ovulation bears little relationship to alterations in sexual behaviour. The bitches were killed and reproductive tracts were removed at various intervals after ovulation and ova or embryos were recovered. The embryo stages were assessed visually and some were investigated histologically. Embryonic development, to early blastocyst stage, took place within the oviducts during the first 12 days after ovulation and there was a marked increase in size between the early and late blastocyst. A culture system using cells from the uterine tube supported the development of one 1-cell embryo to the morula stage.

Keywords: bitch; ovulation; fertilization; embryonic development