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  • Author: MARIA LUISA FORCELLEDO x
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MANUEL DE LA LASTRA and MARÍA LUISA FORCELLEDO

Summary.

Injections of l-vasopressin enhanced the ovulatory response of immature rats to PMSG. This type of ovulation occurred in two phases during the second and third periods of darkness following priming with PMSG. The number of ova collected at the end of each period was increased by l-vasopressin injected either at 28 or at 51 hr, respectively, after the priming. This effect of vasopressin was demonstrated only in rats which were at least 26 days old.

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M. de la LASTRA, MARIA LUISA FORCELLEDO and J. M. STEWART

Vasopressin increases the ovulatory response to PMSG in immature rats (de la Lastra & Forcelledo, 1970). This effect of vasopressin on ovulation, as well as the similarity between the three terminal amino acids of arginine-vasopressin (AVP) and LH-FSH releasing hormone (LH-FSH-RH), induced us to study the influence of the peptide, Pro-Arg-Gly-NH2 (PAGA), which is the terminal fragment of AVP, on the ovulation induced in adult rats with LH or LH-FSH-RH.

Both LH-FSH-RH and PAGA were synthesized by the solid-phase method, using standard techniques (Stewart & Young, 1969). The normal chloromethylated 1% cross-linked polystyrene was used as support, and α-amino groups were blocked with the tert-butyloxycarbonyl group. Toluenesulphonyl blocking groups were used for histidine- and arginine-side