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MARY HAMMOND, M. A. BOONE and B. D. BARNETT

Summary.

This study was undertaken to determine some of the biochemical components of fowl seminal plasma. The semen used in this investigation was collected by artificial ejaculation of 18-month-old White Plymouth Rock males.

Glucose concentration was 74·2±7·8 mg glucose/100 ml of cock seminal plasma. Several enzyme analyses were conducted, using incubation, colorimetry and titration techniques. The enzyme concentrations found were as follows: acid phosphatase, 106±6·5 sjr units; alkaline phosphatase, 6·7±0·83 sjr units; glutamic pyruvic transaminase, 12·8 ±1·4 GPT units; glutamic oxalic transaminase, 560 ±69·6 got units; lactic dehydrogenase, 4610 ±251 ldh units and leucine amino peptidase, 2474 ±436 lap units. Lipase was not found to be present under the conditions of these analyses. Nitrogen concentration as determined by micro-Kjeldahl method averaged 180 mg/100 ml of fowl seminal plasma. Free amino acids found were glutamic acid, glycine, arginine, alanine, serine and methionine. Bound amino acids found were α- or β-alanine, serine, threonine, valine, methionine, tryptophan, leucine (isoleucine, norleucine or leucine) aspartic and glutamic acids, arginine, lysine, histidine, cystine, phenylalanine tyrosine, proline and several unidentified spots.

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MARY HAMMOND, M. A. BOONE, B. D. BARNETT and D. E. TURK

Summary.

The deoxyribonucleic acid of fowl spermatozoa was studied by biochemical means. The nucleotide bases adenine, thymine, guanine and cytosine were found. dna isolated from fowl spermatozoa contained 71 μg adenine and 55 μg thymine/mg. Guanine and cytosine were found, but quantitative results are not reported because of great variability.

The dna isolated from fowl spermatozoa averaged 7·20% phosphorus and 9·45% nitrogen.

Each spermatozoon contained 1·44 × 10-6 mg of nitrogen.