This study was undertaken to determine some of the biochemical components of fowl seminal plasma. The semen used in this investigation was collected by artificial ejaculation of 18-month-old White Plymouth Rock males.
Glucose concentration was 74·2±7·8 mg glucose/100 ml of cock seminal plasma. Several enzyme analyses were conducted, using incubation, colorimetry and titration techniques. The enzyme concentrations found were as follows: acid phosphatase, 106±6·5 sjr units; alkaline phosphatase, 6·7±0·83 sjr units; glutamic pyruvic transaminase, 12·8 ±1·4 GPT units; glutamic oxalic transaminase, 560 ±69·6 got units; lactic dehydrogenase, 4610 ±251 ldh units and leucine amino peptidase, 2474 ±436 lap units. Lipase was not found to be present under the conditions of these analyses. Nitrogen concentration as determined by micro-Kjeldahl method averaged 180 mg/100 ml of fowl seminal plasma. Free amino acids found were glutamic acid, glycine, arginine, alanine, serine and methionine. Bound amino acids found were α- or β-alanine, serine, threonine, valine, methionine, tryptophan, leucine (isoleucine, norleucine or leucine) aspartic and glutamic acids, arginine, lysine, histidine, cystine, phenylalanine tyrosine, proline and several unidentified spots.