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  • Author: N. C. VERA CRUZ x
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D. C. KRAEMER and N. C. VERA CRUZ

The specific gravity (sp. gr.) of baboon spermatozoa was determined using the method described by Danon & Marikovsky (1964) for the determination of the density distribution of red blood cells and used by Lavon, Volcani, Amir & Danon (1966) for sp. gr. determination of bull spermatozoa and seminal plasma. Baboon semen was collected by electro-ejaculation using a rectal probe (Kraemer & Vera Cruz, 1969) and the ejaculate was fractionated within 5 min of ejaculation into a liquid and a coagulum fraction. Only the liquid fraction of the ejaculate was used for sp. gr. determinations. A battery of phthalate ester separating fluids of increasing sp. gr. (1·040 to 1·111) at increments of 0·002 to 0·003 was prepared. A column (10 mm) of separating fluid was drawn into capillary tubes
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D. C. KRAEMER and N. C. VERA CRUZ

This report deals with some gross characteristics of baboon (Papio sp.) semen ejaculated spontaneously or collected by electro-ejaculation. Details of the equipment used and the collection procedure are described and the results of freezing trials are presented. The animals were sedated with Sernylan (Parke, Davis and Co.) at a dose of 0·8 mg/kg intramuscularly. They were then tied in an extended position while lying on their side on the collection table. The penis was cleaned with a mild detergent and then washed thoroughly with water. It was during this process that one of the males ejaculated on three occasions. These ejaculates are referred to as spontaneous ejaculates. The stimulator used was a trans-jector (Nicholson, Inc.) in which the voltage output was controlled by a regulator switch with Steps 1 to 7, with each step having a maximum voltage output of 2·4, 3·2, 4·5, 7·5, 9·5, 10·5 and 13·5 volts, respectively, and a