Exchange of the inert gases 85krypton and 133xenon between the testis and the caput and corpus epididymidis on the ipsilateral but not on the contralateral side has recently been demonstrated in rats. The exchange was probably based on a countercurrent exchange of the gases between the internal spermatic vein and artery (Einer-Jensen, 1974). To investigate if testosterone could be transferred by the same mechanism, the following experiment was performed.
Twenty-four 5- to 6-month-old Charles River male rats were used for the experiment. They were previously used for mating purposes about twice a week.
The animals were anaesthetized with an intraperitoneal injection of 30 mg Mebumal-Na (Nembutal®)/kg. The abdomen was opened through a midline incision, the right testis and the epididymis were withdrawn into the abdomen for injection of 0·2 μg (30 μCi) [1,2-3H]testosterone (N) (New England Nuclear, NET 187) dissolved in 0·1 ml ethyl alcohol benzene