Summary. Human placental protein 14 (PP14) has been purified in high yield from first trimester decidual cytosol. High-performance liquid chromatography on anion exchange, gel filtration and reverse-phase chromatography were used. The protein obtained is approximately 97% pure with an overall recovery of about 50% from the original tissue extract. The first 24 amino acids of the N-terminal were found to be Met-Asp-Ile-Pro-Gln-Thr-Lys-Gln-Asp-Leu-Glu-Leu-Pro-Lys-Leu-Ala-Gly-Thr-Glu-His-Glu-Met-Ala-Met. PP14 has been characterized in this study to be a dimeric glycoprotein of M r 60 000, with homologous subunits having an M r of 28 000.
Keywords: human; PP14; decidual cytosol; h.p.l.c. techniques