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STANLEY S. SHAPIRO, NORMAN E. BROWN and ALLAN S. YARD

Summary.

A high molecular weight acidic glycoprotein has been isolated from rabbit oviducal fluid. Electrophoretic analysis of this purified glycoprotein showed one major component. Antibodies to the glycoprotein were raised in the goat, concentrated by ammonium sulphate precipitation and conjugated with fluorescein isothiocyanate. The conjugated antibodies reacted with 3-day-old rabbit eggs causing them to fluoresce green in u.v. light, but 4-day-old eggs, which have largely lost their coat, did not react significantly with the antibody. These observations suggest that the high molecular weight acidic glycoprotein in oviducal fluid participates in the egg-coating process.