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P. L. PEARSON and M. BOBROW

It has been previously reported that the distal half of the long arm of the human Y chromosome shows a differential affinity for fluorescent acridine derivatives (Zech, 1969; Pearson, Bobrow & Vosa, 1970). By the use of this staining reaction, the Y chromosome can be detected in interphase nuclei of lymphocytes, cultured skin fibroblasts and buccal mucosal cells. This technique has also been used to demonstrate that the X chromosome pairs with the short arm of the Y in first meiotic prophase (Pearson & Bobrow, unpublished observation). This paper describes the appearance of the Y chromosome in the different stages of spermatogenesis.Cytological preparations for the examination of meiotic cells were prepared from testicular tissue of three normal adult males by the method of Evans, Brecon & Ford (1964). Some material was prepared without hypotonic treatment, particularly
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J. P. M. GERAEDTS and P. L. PEARSON

Summary.

The positions of chromosomes 1 and Y inside human spermatozoa were determined by differential staining techniques. In 85/100 cells the two chromosomes were in close contact and in association with a vacuole. This observation is in contrast to previous findings for chromosome No. 9 and the Y-chromosome whose positions do not appear to be correlated.

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A. D. TATES, P. L. PEARSON and J. P. M. GERAEDTS

Department of Radiation Genetics and Chemical Mutagenesis, and Department of Human Genetics, Medical Faculty, University of Leiden, The Netherlands

(Received 9th August 1974)

The introduction of a quinacrine staining technique for identifying human chromosomes (Zech, 1969) also permitted recognition of the human Y chromosome in non-dividing cells (Pearson, Bobrow & Vosa, 1970), including spermatozoa (Pearson & Bobrow, 1970; Barlow & Vosa, 1970). The ability to distinguish between human X- and Y-bearing spermatozoa has been used in estimating the difference in the DNA content of these spermatozoa (Sumner, Robinson & Evans, 1971; Pearson, Geraedts & Pawlowitzki, 1973), the primary non-disjunctional frequency of the Y chromosome (Sumner, Robinson & Evans, 1971; Pawlowitzki & Pearson, 1972) and, more recently, for judging the success of a separation technique for X and Y spermatozoa (Ericsson, Langevin & Nishino, 1973). Chromosomes 1 and 9 have also been recognized in human spermatozoa (Pearson, 1972; Bobrow, Madan &