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  • Author: PHILIP J. DZIUK x
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PHILIP J. DZIUK and H. W. NORTON

Summary.

Rabbits and boars were used to study the effect on the ejaculation process of various drugs known to affect the autonomic nervous system. Of those tried, only atropine had an effect, causing linear reduction, as the dose increased, of the logarithm of the number of spermatozoa ejaculated by rabbits. In the boar, the volume of semen was reduced, but at the dose levels employed total spermatozoa per ejaculate changed little. This suggests that the male accessory sex glands are partly controlled by the parasympathetic system and that this system also has an influence on spermatozoon movement from the epididymis through the vas deferens.

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PHILIP J. DZIUK and MEREDITH N. RUNNER

Summary.

Prepuberal female mice were hormonally induced to ovulate and were artificially inseminated with spermatozoa that had been stored in vitro from 2 to 24 hr. No difference was found in fertilizing ability due to storing spermatozoa for different periods of time. Inseminations were performed at various times relative to ovulation, ranging from 2 hr before ovulation to 13 hr after ovulation. The time of insemination relative to ovulation did influence the percentage of ova that were blastocysts and it appeared that the optimum time for insemination is about 6 hr after ovulation. Blastocysts were recovered from females following insemination up to 13 hr after ovulation. Blastocysts were capable of implantation and developing to 18-day foetuses under the stimulus of progesterone administered orally to the mice. Blastocysts produced by artificial insemination of prepuberal mice are capable of implantation and development after a delay in implantation of at least 11 days. Further refinements of the techniques described may permit more detailed study of the viability of germ cells produced and handled under a wide variety of conditions.