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  • Author: R. J. KOMAREK x
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J. J. LUCAS, B. W. PICKETT and R. J. KOMAREK

Summary.

Semen records of ten ejaculates from each of ten Yorkshire boars were used to estimate the boar and ejaculate variance components for eleven semen characteristics. These data were used to estimate the power of the test. In ten of eleven characteristics, a minimum of five boars and five ejaculates per boar provided at least a 90% chance of detecting a difference of 50% of the mean. For these same ten characteristics, a minimum of fourteen boars and forty-three ejaculates per boar provided at least a 90% chance of detecting a difference of 25% of the mean.

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R. J. KOMAREK, B. W. PICKETT, E. W. GIBSON and R. G. JENSEN

Summary.

In forty-one ejaculates from six boars totalling 7·5 1. the spermatozoa, analysed on an individual ejaculate basis where possible, contained an average of 12·0% lipid which was composed of phospholipids (74·7%), cholesterol (12·6%), diglycerides (5·7%), triglycerides (4·5%) and wax esters (2·4%). Only a small portion of the dry matter of seminal plasma was lipid (0·23%), which was composed of phospholipids (64·7%), cholesterol (17·7%), diglycerides (5·8%), triglycerides (5·3%) and wax esters (6·5%). The gel fraction contained 0·32% lipid, composed of 51·6% phospholipid, 28·3% cholesterol, 6·3% diglycerides, 6·0% triglycerides and 7·8% wax esters.

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R. J. KOMAREK, B. W. PICKETT, E. W. GIBSON and R. N. LANZ

Summary.

Twenty-nine ejaculates of semen were collected from five stallions (four thoroughbreds and one quarterhorse) for this study. The volume of semen, cell count and gel volume/ejaculate averaged 105 ml, 143 × 106/ml and 36·8 ml, respectively. Dry matter content of semen was 3·00%, gel 2·84% and seminal plasma 2·77%; while micrograms of dry matter/106 cells averaged 19·8. The lipids from spermatozoa, seminal plasma and gel from each ejaculate were extracted, and the pooled lipid samples of each fraction were analysed quantitatively for the major lipid classes. Spermatozoa contained 14% lipid, while the seminal plasma and gel contained 2·07% and 2·10%, respectively. The spermatozoal lipid contained phospholipid (58·7%), cholesterol (13·7%), diglycerides (9·0%), triglycerides (8·0%), and wax esters (10·6%). The seminal plasma and gel contained the same lipid classes and the percentages of each were in the same order as above, for seminal plasma: 74·4, 12·1, 3·6, 3·2 and 6·7; for gel: 75·0, 10·0, 5·3, 4·3 and 5·4.