The technique of electro-ejaculation is now commonly used for the collection of semen from some domesticated species and is currently being applied in this Institute to exotic species of differing sizes. This note describes the construction of several types of rectal electrodes which we are using in the development of semen collection techniques. Originally a single rectal pole in combination with a needle pole inserted in the lumbar muscles was used by Gunn (1936) for electro-ejaculation of the ram. Laplaud & Cassou (1945) did not describe the bipolar rectal electrode which they used on rams but later Thibault, Laplaud & Ortavant (1948) gave details of a multipolar electrode having thirty brass rings, separated by vulcanite rings, for use on bulls. Blackshaw (1954) described a four-electrode pole for rams made from copper tubing covered with
P. HEALEY and R. M. F. S. SADLEIR
D. W. RICHARDSON and R. M. F. S. SADLEIR
At concentrations varying from 2·5% to 10·0%, glycerol, dimethyl sulphoxide, ethylene glycol, methyl formamide and methyl acetamide had approximately equally toxic effects on human spermatozoa; whereas dimethyl formamide and dimethyl acetamide were considerably more toxic at the same concentrations.
Glycerol and ethylene glycol afforded an equal protective action to human spermatozoa during freezing and thawing, approximately 50% of the original motility being retained, but negligible protection was exhibited by methyl formamide, dimethyl formamide, methyl acetamide and dimethyl acetamide. Only 34% of the original motility was retained when human spermatozoa were frozen in the diluent containing dimethyl sulphoxide.
I. W. ROWLANDS and R. M. F. S. SADLEIR
Three lionesses of different ages were injected intramuscularly with 1600 i.u. pmsg and, 3 days afterwards, with 1000 i.u. hcg. The reproductive organs were examined 2 days later; eggs were recovered from the Fallopian tubes of two of the animals, one of which had superovulated. The ovaries of the third, much younger lioness contained numerous intact follicles.
The endometrium of the two lionesses which had ovulated contained numerous glands with some degree of coiling and the vaginal epithelium was cornified. The vaginal smear of the un-ovulated animal indicated a pro-oestrous condition.