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R. GIBBONS, K. COLLIS, and R. SELLWOOD

Bovine spermatozoa have been shown to metabolize exogenous substrates both aerobically and anaerobically (Mann, 1964). Fructose (Mann, 1946) and sorbitol (King & Mann, 1958) have been unequivocally identified in seminal plasma, but evidence for the presence of metabolizable substrates in the cervical mucus rests on much less secure foundations. A number of reports that glucose is present in cervical mucus have appeared and indeed Doyle (1958) proposed to use the presence of glucose in human cervical mucus as an index of ovulation. This test has not attained widespread acceptance although there is a general impression that the sugar is present (see e.g. Moghissi, 1973).

The original authority for the presence of glucose in bovine cervical mucus at oestrus is Olds & VanDemark (1957) who used, as others have done since, measurements of reducing value

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R. A. GIBBONS, L. J. BOYD, S. N. DIXON, JANICE PARKER, R. SELLWOOD, and J. B. TASKER

Summary.

Samples of pooled cervical mucus from groups of animals fed melengestrol acetate (MGA) for 14 days were taken 3 and 4 days after cessation of treatment. The mucus samples were separated into protein and glycoprotein portions and the epithelial glycoprotein was isolated. The protein fraction was examined by disc electrophoresis and the epithelial glycoprotein was characterized by chemical analysis and determination of the molecular weight. The cervical mucus of one of the treated groups yielded a glycoprotein which had a reduced sulphate content when compared with that of the controls and did not show the lowered neuraminic acid content characteristic of the glycoprotein from untreated animals at oestrus. Two other groups of MGA-treated animals which received HCG 3 days after withdrawal of the progestagen did not show these anomalies.

The protein in the MGA-treated samples of mucus contained considerably larger amounts of material migrating in the transferrin region of the electrophoretogram than did comparable controls.