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S. BARONOS

Summary.

Galactose has been identified as the main bound carbohydrate of bovine seminal plasma. It was detected chromatographically and determined enzymatically (by galactose dehydrogenase) in acidhydrolysed material. Bound galactose was shown to occur mainly in the non-dialysable portion of bovine seminal plasma in amounts varying from 30 to 150 mg/100 ml seminal plasma. It occurs in a protein-bound form, and is secreted by the seminal vesicles.

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S. BARONOS

The seminal plasma of the boar is known to be rich in inositol but has a much lower content of fructose than that of either the bull or the ram, while stallion seminal plasma contains some inositol and hardly any fructose (Mann, 1954, 1964; Mann, Minotakis & Polge, 1963). The purpose of the present study was to ascertain whether other carbohydrates, either free or bound, occur in boar and stallion seminal plasma.

Semen was collected by artificial vagina and the gel was removed. The seminal plasma, obtained by centrifugation at 2000 g for 10 min, was separated into a dialysable and non-dialysable portion by dialysis at 2 to 5° C against glass-distilled water (five changes at 12-hr intervals). Both the dialysable and non-dialysable portions were concentrated to the original volume of seminal plasma. For the analysis of free

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J. C. BOURSNELL, S. BARONOS, P. A. BRIGGS and E. J. BUTLER

Summary.

Forty-five samples of seminal plasma obtained from eight boars and seventeen samples of vesicular secretion collected from individual boars had mean zinc contents of 22·7 and 137·4 μg/ml, respectively. It was concluded that most of the zinc present in the seminal plasma of the boar is derived from the vesicular secretion.

The mean concentrations of the organic constituents studied were:

Seminal plasma: total nitrogen 4·42 mg/ml (non-dialysable 88·7%), citrate 1·28 mg/ml.

Vesicular secretion: total nitrogen 15·1 mg/ml (non-dialysable 93·9%), citrate 6·07 mg/ml, fructose 0·557 mg/ml, galactose 0·092 mg/ml.

In both seminal plasma and vesicular secretion, the concentration of zinc was positively correlated with that of total and non-dialysable nitrogen and citrate. The correlation with nitrogenous substances was particularly strong in the seminal plasma of individual animals and there was evidence from the partial correlation coefficients that these associations were independent of the zinc-citrate relationship. However, fructose and galactose which do not have an appreciable affinity for zinc under physiological conditions also showed significant positive correlations with zinc (in vesicular secretion) and most of the organic constituents were interrelated in this way, indicating that in such data the influence of chemical combination may be masked by that of other factors which regulate the composition of these fluids.