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R. P. DONAHUE and S. STERN

Recently, Biggers, Whittingham & Donahue (1967) demonstrated that cells liberated from mouse ovarian follicles can support oocyte maturation in vitro. This maturation involves progression from late prophase (germinal vesicle stage) of the first meiotic division to metaphase of the second meiotic division (metaphase II). Oocyte maturation occurred in vitro when cultured either with follicular cells in a glucose medium or without the cells but in the presence of pyruvate or oxaloacetate. These observations suggest that follicular cells cultured in a glucose medium may be forming pyruvate and/or oxaloacetate. In this paper we have measured the amount of pyruvate produced by these cells.

Oocytes and follicular cells from 8- to 15-week-old CF-1 mice (Carworth Inc.) were liberated from their follicles

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S. STERN and A. W. SCHUETZ

Summary.

Adult mice, chosen without regard to the stage of the oestrous cycle, were injected with pmsg. This treatment resulted in ovulation in 77·9% of the animals, within 20 hr. The animals, however, did not mate in this time period. fsh or lh also induced ovulation in this time period.

These results are discussed in their relationship to normal ovulation and mating behaviour in the mouse.