Summary. Treatment with RMI 12,936 blocked the mitotic shift from epithelial to stromal cells normally observed in the uterus on Day 4 of pregnancy, and inhibited implantation. While progesterone alone could reverse the change in pattern of cell division in the preparation of the uterus for implantation, both oestradiol and progesterone were necessary to induce implantation and maintain pregnancy. A reduction in the activity of Δ5-3 β-hydroxysteroid dehydrogenase in the ovaries of RMI 12,936-treated mice suggests that the compound affects luteal synthesis of oestrogen and progesterone in mice.
Geeta Parthasarathy and Safia R. Munshi
SAFIA R. MUNSHI and O. NILSSON
Rabbit antiserum to ovine LH is capable of neutralizing the endogenous activity of mouse LH (Munshi & Rao, 1967). Administration to mated female mice on any one day from Days 2 to 7 after mating results in the inhibition of pregnancy. Since this inhibition can be overcome only by the continuous administration of progesterone, it has been suggested that the antiserum blocks pregnancy by inhibiting progesterone synthesis by the corpus luteum (Munshi, Purandare & Rao, 1972). These studies, however, do not indicate at what stage pregnancy is affected.
Since the various stages of pregnancy differ morphologically, microscopy has been used to examine the early stages of pregnancy in mice injected with antiserum to LH in order to find out when pregnancy is affected.
Random-bred albino mice of the NMRI strain were used. Females were placed with males overnight and examined for the presence of a vaginal plug the next morning.
Tarala D. Nandedkar and Safia R. Munshi
Summary. A single injection of a 5α-dihydrotestosterone (DHT) was administered to cyclic female mice on the day of metoestrus. DHT (1 mg) prolonged the dioestrous stage of the cycle by 24 h and there were 50% fewer large (normal) follicles than in oil-injected controls. Degenerating ova were observed in the oviduct of 70% of the DHT-treated mice and there was a significant reduction in the numbers of females becoming pregnant and bearing normal fetuses.
SAFIA R. MUNSHI, TARALA V. PURANDARE and SHANTA S. RAO
The effect of an antiserum to ovine lh was studied on implantation and deciduoma formation in mice. Administration of the lh antiserum on Day 4 of pregnancy and pseudopregnancy inhibited, respectively, the implantation and decidual cell reaction. The administration of progesterone at the same time as the antiserum overcame the inhibitory effects of the antibodies to lh. The results of these studies indicate that the anti-implantation activity of the lh antiserum is manifest by its inhibition of progesterone synthesis by the cl.
Vijaya Raghavan, Tarala V. Purandare, A. R. Sheth and Safia R. Munshi
The circulating levels of gonadotrophins in immature and adult rats have been studied by several workers (Weisz & Ferin, 1970; Goldman, Grazia, Kamberi & Porter, 1971; Ojeda & Ramirez, 1972; Dohler & Wuttke, 1974, 1975). We have shown that administration of antigonadotrophic sera to neonatal mice affects ovarian development (Purandare, Munshi & Rao, 1976). In the present study the changes in gonadotrophin levels after treatment of neonatal mice wth such antisera were examined.
Geeta Parathasarathy, Tarala Purandare, Bakhtawar Katrak, H. S. Juneja and Safia R. Munshi
Summary. Mice injected with normal rabbit serum on Day 4 of gestation showed a progressive increase in specific alkaline and acid phosphatase activities on Days 6 and 7. Mice injected with antiserum to LH on Day 4 showed a significant decrease in specific alkaline phosphatase activity estimated biochemically on Days 6, 7 and 8, but this decrease was histochemically evident only on Day 8. Similarly, acid phosphatase activity in antiserum-treated mice was significantly increased on Days 6, 7 and 8 when estimated biochemically, but only on Day 8 when studied histochemically.
TARALA V. PURANDARE, B. DATTATREYMURTY, SAFIA R. MUNSHI and SHANTA S. RAO
Neutralization of pituitary prolactin by the administration of antiserum to prolactin in the mouse selectively blocked the pro-oestrous stage of the oestrous cycle and inhibited ovulation in normal cyclic mice. In pseudopregnant mice, the antiserum shortened the duration of pseudopregnancy. Failure of the antiserum to affect uterine cell division, blastocyst implantation and pregnancy suggests that the secretion of progesterone by the luteal tissue appears to be more influenced by LH than by prolactin.