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SIDNEY SHULMAN and PAUL BRONSON

Summary.

It was found that human seminal plasma contained a large number of antigenic components as tested with selected antiserum bleedings from rabbits that had been intensively immunized with pooled seminal plasma. Gel diffusion experiments revealed a total of eight to eleven distinctive components, although several experiments had to be considered jointly to achieve unambiguous resolution. These experiments included variations in the concentration of the seminal plasma and the spacings between the wells.

Immuno-electrophoresis gave a clearer resolution. A characteristic pattern of twelve components was seen, consisting of four major mobility groups of antigens with high diffusion rates and two other mobility groups with low diffusion rates. These were termed A, B1, B2, C1, C2, C3, D1, D2, E1, E2, F1 and F2. It was further shown that serum albumin was one of the components in seminal plasma (component A).

It was also found that the predominant electrophoretic component could be isolated by starch block electrophoresis, and that this corresponded to component C2 of the immuno-electrophoretic pattern.

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SIDNEY SHULMAN and JOANNA M. FERBER

Summary.

Human prostatic fluid and prostate extract were examined for prostatic acid phosphatase by means of gel diffusion precipitation, using rabbit antisera. Specific staining for phosphatase was performed on the antigen—antibody precipitation lines. Three major and two minor bands, staining for phosphatase, were detected: one of the major bands was shown to correspond to a non-specific precipitate. Of the four specific precipitates, one was found only for fluid, but not for extract. The staining of all of the precipitates was inhibited by the presence of tartrate, confirming the identity of the antigen as prostatic-type phosphatase.

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SIDNEY SHULMAN, LOUISE MAMROD, RAYMOND W. LANG, MAURICE J. GONDER and WARD A. SOANES

Summary.

Prostatic acid phosphatase can be detected in individual human prostatic fluids by means of gel diffusion antigen-antibody precipitation with specific staining procedures. The fluids can be diluted to various degrees before the reaction fails. This titre, or limiting dilution, ranged from 1 : 64 to 1 : 16,000. It paralleled the results obtained by the King-Armstrong assay of enzyme activity. Gel diffusion analysis revealed the presence of two antigens with acid phosphatase activity.