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  • Author: STANLEY S. SHAPIRO x
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STANLEY S. SHAPIRO, JERILYN P. JENTSCH and ALLAN S. YARD

Summary.

The protein composition of rabbit oviducal fluid was studied with the aid of disc gel electrophoresis and immunological techniques. While many proteins of the oviducal fluid were similar to those in the serum, some different proteins were only present in the fluid. After immunization with yeast alcohol dehydrogenase of a rabbit fitted with an oviducal fluid collecting device, antibody to the dehydrogenase was found in the serum but not in the oviducal fluid. The results of these studies on the protein composition of rabbit oviducal fluid suggest that the fluid arises from both transudation and secretion.

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STANLEY S. SHAPIRO, NORMAN E. BROWN and ALLAN S. YARD

Summary.

A high molecular weight acidic glycoprotein has been isolated from rabbit oviducal fluid. Electrophoretic analysis of this purified glycoprotein showed one major component. Antibodies to the glycoprotein were raised in the goat, concentrated by ammonium sulphate precipitation and conjugated with fluorescein isothiocyanate. The conjugated antibodies reacted with 3-day-old rabbit eggs causing them to fluoresce green in u.v. light, but 4-day-old eggs, which have largely lost their coat, did not react significantly with the antibody. These observations suggest that the high molecular weight acidic glycoprotein in oviducal fluid participates in the egg-coating process.