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H. ADLERCREUTZ and T. LUUKKAINEN

Summary.

Free 17α-oestradiol has been isolated and identified in a pregnant cow's bile. The identification was based on several specific colour reactions, the behaviour of different derivatives of the compound in alumina and thin-layer chromatography and finally on a great number of gas chromatographic investigations. A sensitive and highly specific colour test for 17α-oestradiol and its derivatives in thin-layer chromatograms, making use of phosphomolybdic acid, has been presented.