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Liu Shi-fan, Wang Zhong-xing, Yuan Yao-e, Bing Sheng-min, Zhang Bei-zhu, Wu Jin-zhi, Wu Yi-e, and Peng Xin-ying

Summary. The concentrations of LH, FSH, prolactin, oestradiol and progesterone in serum were measured daily during the menstrual cycle of 100 normal Chinese women. The cyclic changes in LH, FSH, oestradiol and progesterone were typical of ovulatory cycles in women of other ethnic groups as reported in the literature. The geometric mean of the LH midcycle peak value was 51·64 i.u./l, the FSH mid-cycle peak value was 11 ·52 i.u./l, the preovulatory oestradiol peak was 1229·12 pmol/1, and the progesterone luteal maximum was 53·27 nmol/1. The cyclic changes of prolactin concentrations were irregular: the value at mid-cycle was significantly higher than that at the follicular or luteal phases.

A correlation between the length of the cycle and mean concentrations of LH and oestradiol at different stages throughout the cycle was shown.

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Zi-gang Shen, Wei He, Ji Zhang, Hai-yang He, Xia Yang, Zheng-qiong Chen, Ping Yang, Jian Li, Zhi-qing Liang, Yu-zhang Wu, and Jin-tao Li

SPINLW1 (previously known as eppin (epididymal protease inhibitor)) is a target under intense scrutiny in the study of male contraceptive vaccines. B-cell-dominant epitopes are now recognized as key parts of the induction of humoral immune responses against target antigens. The generation of robust humoral responses in vivo has become a crucial problem in the development of modern vaccines. In this study, we developed a completely novel B-cell-dominant-epitope-based mimovirus vaccine, which is a kind of virus-size particulate antigen delivery system. The mimovirus successfully self-assembled from a cationic peptide containing a cell-penetrating peptide of TAT49–57 and a plasmid DNA encoding both three SPINLW1 (103–115) copies and adjuvant C3d3. The male mice were immunized with the epitope-based mimovirus vaccine, which resulted in a gradual elevation of specific serum IgG antibody levels. These reached a peak at week 4. Mating for the fertility assay showed that the mimovirus vaccine had accomplished a moderate fertility inhibition effect and investigation into the mechanism of action showed that it did so by interfering with the reproductive function of the sperm but that it did not damage the structures of the testes or cause serum testosterone to decline. Our results suggest an ideal protocol for suppressing fertility in mice by an engineered mimovirus vaccine.