Search Results

You are looking at 1 - 2 of 2 items for

  • Author: Xinyue Wang x
  • Refine by Access: All content x
Clear All Modify Search
Nan Meng NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

Search for other papers by Nan Meng in
Google Scholar
PubMed
Close
,
Xinyue Wang Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China

Search for other papers by Xinyue Wang in
Google Scholar
PubMed
Close
,
Yan Shi NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

Search for other papers by Yan Shi in
Google Scholar
PubMed
Close
,
Yanyan Mao NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

Search for other papers by Yanyan Mao in
Google Scholar
PubMed
Close
,
Qian Yang NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

Search for other papers by Qian Yang in
Google Scholar
PubMed
Close
,
Baohui Ju Department of Gynecology and Obstetrics, The Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of China

Search for other papers by Baohui Ju in
Google Scholar
PubMed
Close
,
Qianxi Zhu NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

Search for other papers by Qianxi Zhu in
Google Scholar
PubMed
Close
,
Tingting Zhang Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China

Search for other papers by Tingting Zhang in
Google Scholar
PubMed
Close
,
Yan Gu Department of Gynecology and Obstetrics, The Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of China

Search for other papers by Yan Gu in
Google Scholar
PubMed
Close
, and
Xuan Zhang NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

Search for other papers by Xuan Zhang in
Google Scholar
PubMed
Close

Decidualization is essential for the successful establishment of pregnancy, and the dysregulated decidualization may lead to early pregnancy loss. It was previously reported by us that miR-3074-5p could promote apoptosis but inhibit invasion of human extravillous trophoblast (EVT) cells in vitro, and the expression level of miR-3074-5p in villus tissues of recurrent miscarriage (RM) patients was significantly increased. The aim of this study was to preliminarily explore the role of miR-3074-5p played in the decidualization of human endometrial stromal cells (ESCs). It was found that the decidual expression level of miR-3074-5p in RM patients was remarkably higher than that in the control group. The overexpression of miR-3074-5p in the immortalized human ESC line, T-HESCs, showed suppressive effects not only on the cell proliferation, as well as the intracellular expression levels of cyclin B1 (CCNB1), CCND1 and CCNE1 but also on the in vitro-induced decidualization. CLN8 mRNA, encoding an endoplasmic reticulum (ER)-associated membrane protein, was validated to be directly targeted by miR-3074-5p. And, the expression level of CLN8 was continuously increased along with the decidualization process, whereas down-regulated CLN8 expression could inhibit the decidualization of T-HESCs in vitro. Furthermore, contrary to the increased expression level of miR-3074-5p, a significantly decreased CLN8 expression was observed in decidual tissues of RM patients. Collectively, these data suggested that an increased miR-3074-5p expression in ESCs might cause early pregnancy failure by disturbing decidualization of ESCs via the miR-3074-5p/CLN8 pathway, providing a potential diagnostic and therapeutic target for RM.

Restricted access
Hong-Jie Yuan Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Hong-Jie Yuan in
Google Scholar
PubMed
Close
,
Zhi-Bin Li Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Zhi-Bin Li in
Google Scholar
PubMed
Close
,
Xin-Yue Zhao Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Xin-Yue Zhao in
Google Scholar
PubMed
Close
,
Guang-Yi Sun Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Guang-Yi Sun in
Google Scholar
PubMed
Close
,
Guo-Liang Wang Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Guo-Liang Wang in
Google Scholar
PubMed
Close
,
Ying-Qi Zhao Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Ying-Qi Zhao in
Google Scholar
PubMed
Close
,
Min Zhang Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Min Zhang in
Google Scholar
PubMed
Close
, and
Jing-He Tan Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Jing-He Tan in
Google Scholar
PubMed
Close

Mechanisms by which female stress and particularly glucocorticoids impair oocyte competence are largely unclear. Although one study demonstrated that glucocorticoids triggered apoptosis in ovarian cells and oocytes by activating the FasL/Fas system, other studies suggested that they might induce apoptosis through activating other signaling pathways as well. In this study, both in vivo and in vitro experiments were conducted to test the hypothesis that glucocorticoids might trigger apoptosis in oocytes and ovarian cells through activating the TNF-α system. The results showed that cortisol injection of female mice (1.) impaired oocyte developmental potential and mitochondrial membrane potential with increased oxidative stress; (2.) induced apoptosis in mural granulosa cells (MGCs) with increased oxidative stress in the ovary; and (3.) activated the TNF-α system in both ovaries and oocytes. Culture with corticosterone induced apoptosis and activated the TNF-α system in MGCs. Knockdown or knockout of TNF-α significantly ameliorated the pro-apoptotic effects of glucocorticoids on oocytes and MGCs. However, culture with corticosterone downregulated TNF-α expression significantly in oviductal epithelial cells. Together, the results demonstrated that glucocorticoids impaired oocyte competence and triggered apoptosis in ovarian cells through activating the TNF-α system and that the effect of glucocorticoids on TNF-α expression might vary between cell types.

Restricted access