Search Results

You are looking at 1 - 8 of 8 items for

  • Author: Yan Gao x
  • Refine by access: All content x
Clear All Modify Search
Lizhu Ma College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A & F University, Yangling, China

Search for other papers by Lizhu Ma in
Google Scholar
PubMed
Close
,
Yuxin Zheng College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A & F University, Yangling, China

Search for other papers by Yuxin Zheng in
Google Scholar
PubMed
Close
,
Xiaorong Tang College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A & F University, Yangling, China

Search for other papers by Xiaorong Tang in
Google Scholar
PubMed
Close
,
Huimin Gao College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A & F University, Yangling, China

Search for other papers by Huimin Gao in
Google Scholar
PubMed
Close
,
Ning Liu College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A & F University, Yangling, China

Search for other papers by Ning Liu in
Google Scholar
PubMed
Close
,
Yan Gao Faculty of Medical and Chemical Engineering, Xianyang Vocational Technical College, Xi’an, China

Search for other papers by Yan Gao in
Google Scholar
PubMed
Close
,
Lizhuang Hao State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinghai Plateau Yak Research Center, Qinhai University, Xining, China

Search for other papers by Lizhuang Hao in
Google Scholar
PubMed
Close
,
Shujie Liu State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinghai Plateau Yak Research Center, Qinhai University, Xining, China

Search for other papers by Shujie Liu in
Google Scholar
PubMed
Close
, and
Zhongliang Jiang College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A & F University, Yangling, China

Search for other papers by Zhongliang Jiang in
Google Scholar
PubMed
Close

It is well documented that granulosa cell apoptosis is the main reason for follicular atresia and death; however, increasing evidence suggests that autophagy plays an important role in the fate of granulosa cells. miR-21-3p regulates many fundamental biological processes and is pivotal in the autophagy of tumor cells; nevertheless, the autophagy in cattle ovary and how miR-21-3p regulates the follicular cells is unknown. In this study, we aimed to elucidate the autophagy and the role of miR-21-3p in cattle ovary using bovine primary ovarian granulosa cells (BGCs). The results showed the autophagy for the first time in BGCs in large follicle according to autophagic gene transcript of LC3, BECN-1, ATG3, protein expression of LC3, P62 and LC3 puncta, a standard marker for autophagosomes. miR-21-3p was identified as a novel miRNA that repressed BGCs autophagy according to the results from plasmids transfection of miR-21-3p mimics and inhibitor. Meanwhile, VEGFA was confirmed to be a validated target of miR-21-3p in BGCs using luciferase reporter assays and the results of VEGFA expression decreased with transfection of miR-21-3p mimics, while it increased with transfection of miR-21-3p inhibitor. In addition, small interference-mediated knockdown of VEGFA significantly inhibits BGCs autophagy signaling; however, overexpression of VEGFA in BGCs promoted autophagy in the presence of miR-21-3p. Finally, the results of AKT and its phosphorylation suggested that miR-21-3p suppressed VEGFA expression through downregulating AKT phosphorylation signaling. In summary, this study demonstrates that miR-21-3p inhibits BGCs autophagy by targeting VEGFA and attenuating PI3K/AKT signaling.

Restricted access
Jiangman Gao Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital,Peking University Third Hospital, Beijing, China
National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China
Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology (Peking University Third Hospital), Beijing, China

Search for other papers by Jiangman Gao in
Google Scholar
PubMed
Close
,
Zhiqiang Yan Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital,Peking University Third Hospital, Beijing, China
National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China
Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology (Peking University Third Hospital), Beijing, China

Search for other papers by Zhiqiang Yan in
Google Scholar
PubMed
Close
,
Liying Yan Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital,Peking University Third Hospital, Beijing, China
National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China
Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology (Peking University Third Hospital), Beijing, China

Search for other papers by Liying Yan in
Google Scholar
PubMed
Close
,
Xiaohui Zhu Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital,Peking University Third Hospital, Beijing, China
National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China
Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology (Peking University Third Hospital), Beijing, China

Search for other papers by Xiaohui Zhu in
Google Scholar
PubMed
Close
,
Hui Jiang Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital,Peking University Third Hospital, Beijing, China

Search for other papers by Hui Jiang in
Google Scholar
PubMed
Close
, and
Jie Qiao Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital,Peking University Third Hospital, Beijing, China
National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China
Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology (Peking University Third Hospital), Beijing, China

Search for other papers by Jie Qiao in
Google Scholar
PubMed
Close

In brief

Whether sperm DNA fragmentation (SDF) affects embryo development and clinical outcomes is still controversial, which limits the utility of SDF testing in assisted reproductive technology management. This study demonstrates that high SDF is associated with the incidence of segmental chromosomal aneuploidy and increased paternal whole chromosomal aneuploidies.

Abstract

We aimed to investigate the correlation of sperm DNA fragmentation (SDF) with the incidence and paternal origin of whole and segmental chromosomal aneuploidies of embryos at the blastocyst stage. A retrospective cohort study was conducted with a total of 174 couples (women aged 35 years or younger) who underwent 238 cycles (including 748 blastocysts) of preimplantation genetic testing for monogenic diseases (PGT-M). All subjects were divided into two groups based on the sperm DNA fragmentation index (DFI) level: low DFI (<27%) and high DFI (≥27%). The rates of euploidy, whole chromosomal aneuploidy, segmental chromosomal aneuploidy, mosaicism, parental origin of aneuploidy, fertilization, cleavage, and blastocyst formation were compared between low- and high-DFI groups. We found no significant differences in fertilization, cleavage, or blastocyst formation between the two groups. Compared to that in the low-DFI group, segmental chromosomal aneuploidy rate was significantly higher in the high-DFI group (11.57% vs 5.83%, P = 0.021; OR: 2.32, 95% CI: 1.10–4.89, P = 0.028). The whole chromosomal embryonic aneuploidy of paternal origin was significantly higher in cycles with high DFI than in cycles with low DFI (46.43% vs 23.33%, P = 0.018; OR: 4.32, 95% CI: 1.06–17.66, P = 0.041). However, the segmental chromosomal aneuploidy of paternal origin was not significantly different between the two groups (71.43% vs 78.05%, P = 0.615; OR: 1.01, 95% CI: 0.16–6.40, P = 0.995). In conclusion, our results suggested that high SDF was associated with the incidence of segmental chromosomal aneuploidy and increased paternal whole chromosomal aneuploidies in embryos.

Open access
Ping Zhou Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian City 271018, Shandong Province, People's Republic of China

Search for other papers by Ping Zhou in
Google Scholar
PubMed
Close
,
Yan-Guang Wu Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian City 271018, Shandong Province, People's Republic of China

Search for other papers by Yan-Guang Wu in
Google Scholar
PubMed
Close
,
Qing Li Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian City 271018, Shandong Province, People's Republic of China

Search for other papers by Qing Li in
Google Scholar
PubMed
Close
,
Guo-Cheng Lan Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian City 271018, Shandong Province, People's Republic of China

Search for other papers by Guo-Cheng Lan in
Google Scholar
PubMed
Close
,
Gang Wang Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian City 271018, Shandong Province, People's Republic of China

Search for other papers by Gang Wang in
Google Scholar
PubMed
Close
,
Da Gao Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian City 271018, Shandong Province, People's Republic of China

Search for other papers by Da Gao in
Google Scholar
PubMed
Close
, and
Jing-He Tan Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian City 271018, Shandong Province, People's Republic of China

Search for other papers by Jing-He Tan in
Google Scholar
PubMed
Close

To improve in vitro maturation (IVM) of denuded oocytes (DOs), we observed the interactive effects of cysteamine, cystine and cumulus cells on the glutathione (l-γ-glutamyl-l-cysteinyl-glycine; GSH) level and developmental capacity of goat IVM oocytes. Cysteamine supplementation increased the GSH level and blastocyst rates of both cumulus–oocyte complexes (COCs) and DOs, while the addition of cystine increased the GSH level and blastulation only in the presence of cumulus cells (COCs or DOs co-cultured on a cumulus cell monolayer). Simultaneous supplementation of cysteamine and cystine increased the GSH content and blastulation of co-cultured DOs to a level similar to that of COCs matured without thiol supplementation. Co-culture without thiol supplementation improved DOs' GSH synthesis but not blastulation. The results suggest that DOs cannot utilize cystine for GSH synthesis unless exogenous cysteamine is supplied by either cumulus cells or supplementation. Thus, while the addition of cystine alone is enough to improve IVM of COCs, improvement of DOs requires supplementation of both cystine and cysteamine. Synergic actions between cysteamine, cystine and cumulus cells restore the GSH level and developmental capacity of goat DOs.

Free access
Hua-Yu Lian College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Hua-Yu Lian in
Google Scholar
PubMed
Close
,
Yan Gao College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Yan Gao in
Google Scholar
PubMed
Close
,
Guang-Zhong Jiao College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Guang-Zhong Jiao in
Google Scholar
PubMed
Close
,
Ming-Ju Sun College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Ming-Ju Sun in
Google Scholar
PubMed
Close
,
Xiu-Fen Wu College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Xiu-Fen Wu in
Google Scholar
PubMed
Close
,
Tian-Yang Wang College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Tian-Yang Wang in
Google Scholar
PubMed
Close
,
Hong Li College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Hong Li in
Google Scholar
PubMed
Close
, and
Jing-He Tan College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People's Republic of China

Search for other papers by Jing-He Tan in
Google Scholar
PubMed
Close

In this study, using a mouse model, we tested the hypothesis that restraint stress would impair the developmental potential of oocytes by causing oxidative stress and that antioxidant supplementation could overcome the adverse effect of stress-induced oxidative stress. Female mice were subjected to restraint stress for 24 h starting 24 h after equine chorionic gonadotropin injection. At the end of stress exposure, mice were either killed to recover oocytes for in vitro maturation (IVM) or injected with human chorionic gonadotropin and caged with male mice to observe in vivo development. The effect of antioxidants was tested in vitro by adding them to IVM medium or in vivo by maternal injection immediately before restraint stress exposure. Assays carried out to determine total oxidant and antioxidant status, oxidative stress index, and reactive oxygen species (ROS) and glutathione levels indicated that restraint stress increased oxidative stress in mouse serum, ovaries, and oocytes. Whereas the percentage of blastocysts and number of cells per blastocyst decreased significantly in oocytes from restraint-stressed mice, addition of antioxidants to IVM medium significantly improved their blastocyst development. Supplementation of cystine and cysteamine to IVM medium reduced ROS levels and aneuploidy while increasing glutathione synthesis and improving pre- and postimplantation development of oocytes from restraint-stressed mice. Furthermore, injection of the antioxidant epigallocatechin gallate into restraint-stressed mice significantly improved the blastocyst formation and postimplantation development of their oocytes. In conclusion, restraint stress at the oocyte prematuration stage impaired the developmental potential of oocytes by increasing oxidative stress and addition of antioxidants to IVM medium or maternal antioxidant injection overcame the detrimental effect of stress-induced oxidative stress. The data reported herein are helpful when making attempts to increase the chances of a successful outcome in human IVF, because restraint was applied at a stage similar to the FSH stimulation period in a human IVF program.

Free access
C L Lu Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by C L Lu in
Google Scholar
PubMed
Close
,
J Yan Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by J Yan in
Google Scholar
PubMed
Close
,
X Zhi Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by X Zhi in
Google Scholar
PubMed
Close
,
X Xia Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by X Xia in
Google Scholar
PubMed
Close
,
T R Wang Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by T R Wang in
Google Scholar
PubMed
Close
,
L Y Yan Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by L Y Yan in
Google Scholar
PubMed
Close
,
Y Yu Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by Y Yu in
Google Scholar
PubMed
Close
,
T Ding Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by T Ding in
Google Scholar
PubMed
Close
,
J M Gao Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by J M Gao in
Google Scholar
PubMed
Close
,
R Li Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by R Li in
Google Scholar
PubMed
Close
, and
J Qiao Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China
Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Center for Reproductive Medicine, Medical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, China

Search for other papers by J Qiao in
Google Scholar
PubMed
Close

Fertility preservation is an important type of frontier scientific research in the field of reproductive health. The culture of ovarian cortices to i) initiate primordial follicle growth and ii) procure developing follicles for later oocyte maturation is a promising fertility preservation strategy, especially for older women or cancer patients. At present, this goal remains largely unsubstantiated in primates because of the difficulty in attaining relatively large follicles via ovarian cortex culture. To overcome this hurdle, we cultured macaque monkey ovarian cortices with FSH, kit ligand (KL), basic fibroblast growth factor (bFGF), and/or epidermal growth factor (EGF). The various factors and factor combinations promoted primordial follicle development to different extents. Notably, both bFF (bFGF, 100 ng/ml and FSH, 50 ng/ml) and KF (KL, 100 ng/ml and FSH, 50 ng/ml) contributed to the activation of primordial follicles at day 12 (D12) of culture, whereas at D18, the proportions of developing follicles were significantly higher in the bFF and KF groups relative to the other treatment groups, particularly in the bFF group. Estradiol and progesterone production were also highest in the bFF group, and primary follicle diameters were the largest. Up until D24, the bFF group still exhibited the highest proportion of developing follicles. In conclusion, the bFGF–FSH combination promotes nonhuman primate primordial follicle development in vitro, with the optimal experimental window within 18 days. These results provide evidence for the future success of human ovarian cortex culture and the eventual acquisition of mature human follicles or oocytes for fertility restoration.

Free access
Lanlan Fang Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Lanlan Fang in
Google Scholar
PubMed
Close
,
Zhen Wang Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Zhen Wang in
Google Scholar
PubMed
Close
,
Ze Wu Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Ze Wu in
Google Scholar
PubMed
Close
,
Yang Yan Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Yang Yan in
Google Scholar
PubMed
Close
,
Yibo Gao Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Yibo Gao in
Google Scholar
PubMed
Close
,
Yuxi Li Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Yuxi Li in
Google Scholar
PubMed
Close
,
Jung-Chien Cheng Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Jung-Chien Cheng in
Google Scholar
PubMed
Close
, and
Ying-Pu Sun Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

Search for other papers by Ying-Pu Sun in
Google Scholar
PubMed
Close

Matrix metalloproteinases (MMPs) play a pivotal role in the regulation of cell invasion. Placental trophoblast cell invasion is a precisely regulated event. Dysregulation of MMPs has been linked to various placental diseases. Growth differentiation factor-8 (GDF-8), also known as myostatin, is a member of the transforming growth factor-beta (TGF-β) superfamily. GDF-8 and its putative receptors are expressed in human extravillous cytotrophoblast cells (EVTs). Although the pro-invasive effect of GDF-8 in human EVT cells has been recently reported, the underlying molecular mechanism remains largely unknown. In this study, we investigate the effects of GDF-8 on the expression of the two most important MMPs, MMP2 and MMP9, in the HTR-8/SVneo human EVT cell line. Our results show that GDF-8 significantly upregulates the expression of MMP2. The expression of MMP9 is not affected by GDF-8. Using a siRNA-mediated knockdown approach, we reveal that the stimulatory effect of GDF-8 on MMP2 expression is mediated by the ALK5-SMAD2/3 signaling pathway. Additionally, the knockdown of MMP2 attenuates the GDF-8-induced cell invasiveness. These findings deepen our understanding of the biological roles of GDF-8 in the regulation of human trophoblast cell invasion.

Restricted access
Qiao-Song Zheng State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Qiao-Song Zheng in
Google Scholar
PubMed
Close
,
Xiao-Na Wang State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Xiao-Na Wang in
Google Scholar
PubMed
Close
,
Qing Wen State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Qing Wen in
Google Scholar
PubMed
Close
,
Yan Zhang State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Yan Zhang in
Google Scholar
PubMed
Close
,
Su-Ren Chen State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Su-Ren Chen in
Google Scholar
PubMed
Close
,
Jun Zhang State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Jun Zhang in
Google Scholar
PubMed
Close
,
Xi-Xia Li State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Xi-Xia Li in
Google Scholar
PubMed
Close
,
Ri-Na Sha State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Ri-Na Sha in
Google Scholar
PubMed
Close
,
Zhao-Yuan Hu State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Zhao-Yuan Hu in
Google Scholar
PubMed
Close
,
Fei Gao State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Fei Gao in
Google Scholar
PubMed
Close
, and
Yi-Xun Liu State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Search for other papers by Yi-Xun Liu in
Google Scholar
PubMed
Close

Spermatogenesis is a complex process involving the regulation of multiple cell types. As the only somatic cell type in the seminiferous tubules, Sertoli cells are essential for spermatogenesis throughout the spermatogenic cycle. The Wilms tumor gene, Wt1, is specifically expressed in the Sertoli cells of the mouse testes. In this study, we demonstrated that Wt1 is required for germ cell differentiation in the developing mouse testes. At 10 days post partum, Wt1-deficient testes exhibited clear meiotic arrest and undifferentiated spermatogonia accumulation in the seminiferous tubules. In addition, the expression of claudin11, a marker and indispensable component of Sertoli cell integrity, was impaired in Wt1 −/flox ; Cre-ER TM testes. This observation was confirmed in in vitro testis cultures. However, the basal membrane of the seminiferous tubules in Wt1-deficient testes was not affected. Based on these findings, we propose that Sertoli cells' status is affected in Wt1-deficient mice, resulting in spermatogenesis failure.

Free access
Muyun Wei Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Muyun Wei in
Google Scholar
PubMed
Close
,
Ying Gao Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Ying Gao in
Google Scholar
PubMed
Close
,
Bingru Lu Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Bingru Lu in
Google Scholar
PubMed
Close
,
Yulian Jiao Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Yulian Jiao in
Google Scholar
PubMed
Close
,
Xiaowen Liu Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Xiaowen Liu in
Google Scholar
PubMed
Close
,
Bin Cui Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Bin Cui in
Google Scholar
PubMed
Close
,
Shengnan Hu Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Shengnan Hu in
Google Scholar
PubMed
Close
,
Linying Sun Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Linying Sun in
Google Scholar
PubMed
Close
,
Shaowei Mao Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Shaowei Mao in
Google Scholar
PubMed
Close
,
Jing Dong Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Jing Dong in
Google Scholar
PubMed
Close
,
Lei Yan Centre for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Lei Yan in
Google Scholar
PubMed
Close
,
Zijiang Chen Centre for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Zijiang Chen in
Google Scholar
PubMed
Close
, and
Yueran Zhao Department of Centre Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China
Centre for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

Search for other papers by Yueran Zhao in
Google Scholar
PubMed
Close

Defective decidualization of human endometrial stromal cells (ESCs) has recently been highlighted as an underlying cause of implantation failure. FK-506-binding protein 51 (FKBP51) has been shown to participate in the steroid hormone response and the protein kinase B (AKT) regulation process, both of which are important pathways involved in decidualization. The objective of the present study was to investigate the potential effects and mechanisms of FKBP51 in the regulation of ESC decidualization. By performing immunohistochemical staining on an endometrial tissue microarray (TMA) derived from normal females, we found that FKBP51 expression was much higher in the luteal phase than in the follicular phase in ESCs. Primary ESCs were isolated from patients to build an in vitro decidualization model through co-culture with medroxyprogesterone acetate (MPA) and 8-bromoadenosine (cAMP). SC79, a specific AKT activator in various physiological and pathological conditions, and shRNA-FKBP51 were used to examine the roles of AKT and FKBP51 in decidualization. The Western blot and RT-PCR results showed that FKBP51, insulin-like growth factor-binding protein 1 (IGFBP1) and prolactin (PRL) expression increased in ESCs treated with MPA + cAMP; meanwhile, the level of p-Ser473 AKT (p-S473 AKT) decreased and forkhead box protein O1 (FOXO1A) expression increased. Decidualization was inhibited by the AKT activator SC79 and the transfection of FKBP51-shRNA by affecting protein synthesis, cell morphology, cell growth and cell cycle. Furthermore, this inhibition was rescued by FKBP51-cDNA transfection. The results supported that FKBP51 promotes decidualization by reducing the Ser473 phosphorylation levels in AKT.

Free access