Search Results

You are looking at 1 - 1 of 1 items for

  • Author: Yanbo Du x
  • Refine by access: All content x
Clear All Modify Search
Xiaoyang Wen Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China
State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China
Medical Integration and Practice Center, Shandong University, Jinan, Shandong, People’s Republic of China
Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, People’s Republic of China
Reproductive Hospital Affiliated to Shandong University, Jinan, Shandong, People’s Republic of China

Search for other papers by Xiaoyang Wen in
Google Scholar
PubMed
Close
,
Jingyang Zhang Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China
State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China
Medical Integration and Practice Center, Shandong University, Jinan, Shandong, People’s Republic of China
Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, People’s Republic of China
Reproductive Hospital Affiliated to Shandong University, Jinan, Shandong, People’s Republic of China

Search for other papers by Jingyang Zhang in
Google Scholar
PubMed
Close
,
Zihan Xu Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China
State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China
Medical Integration and Practice Center, Shandong University, Jinan, Shandong, People’s Republic of China
Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, People’s Republic of China
Reproductive Hospital Affiliated to Shandong University, Jinan, Shandong, People’s Republic of China

Search for other papers by Zihan Xu in
Google Scholar
PubMed
Close
,
Muzi Li Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China
State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China
Medical Integration and Practice Center, Shandong University, Jinan, Shandong, People’s Republic of China
Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, People’s Republic of China
Reproductive Hospital Affiliated to Shandong University, Jinan, Shandong, People’s Republic of China

Search for other papers by Muzi Li in
Google Scholar
PubMed
Close
,
Xiaotong Dong Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China
State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China
Medical Integration and Practice Center, Shandong University, Jinan, Shandong, People’s Republic of China
Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, People’s Republic of China
Reproductive Hospital Affiliated to Shandong University, Jinan, Shandong, People’s Republic of China

Search for other papers by Xiaotong Dong in
Google Scholar
PubMed
Close
,
Yanbo Du Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China
State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China
Medical Integration and Practice Center, Shandong University, Jinan, Shandong, People’s Republic of China
Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, People’s Republic of China
Reproductive Hospital Affiliated to Shandong University, Jinan, Shandong, People’s Republic of China

Search for other papers by Yanbo Du in
Google Scholar
PubMed
Close
,
Zhen Xu Center for Medical Genetics and Prenatal Diagnosis, Shandong Provincial Maternal and Child Health Care Hospital, Jinan, Shandong, China
Shandong Medicine and Health Key Laboratory of Birth Defect Prevention and Genetic Medicine, Jinan, Shandong, China
Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Jinan, Shandong, China

Search for other papers by Zhen Xu in
Google Scholar
PubMed
Close
, and
Lei Yan Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China
State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China
Medical Integration and Practice Center, Shandong University, Jinan, Shandong, People’s Republic of China
Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, People’s Republic of China
Reproductive Hospital Affiliated to Shandong University, Jinan, Shandong, People’s Republic of China

Search for other papers by Lei Yan in
Google Scholar
PubMed
Close

In brief

Abnormal glucose metabolism may be involved in the pathogenesis of endometriosis. The present study identifies that highly expressed H19 leads to increased aerobic glycolysis and histone lactylation levels in endometriosis.

Abstract

Previous studies from our group and others have shown increased IncRNA H19 expression in both the eutopic endometrium and the ectopic endometriosis tissue during endometriosis. In this study, we use immunofluorescence, immunohistochemistry, and protein quantification to determine that levels of aerobic glycolysis and histone lactylation are increased in endometriosis tissues. In human endometrial stromal cells, we found that high H19 expression resulted in abnormal glucose metabolism by examining the levels of glucose, lactate, and ATP and measuring protein levels of enzymes that participate in glycolysis. At the same time, immunofluorescence and western blotting demonstrated increased histone lactylation in H19 overexpressing cells. Altering aerobic glycolysis and histone lactylation levels through the addition of sodium lactate and 2-deoxy-d-glucose demonstrated that increased aerobic glycolysis and histone lactylation levels resulted in enhanced cell proliferation and cell migration, contributing to endometriosis. To validate these findings in vivo, we constructed an endometriosis mouse model, demonstrating similar changes in endometriosis tissues in vivo. Both aerobic glycolysis and histone lactylation levels were elevated in endometriotic lesions. Taken together, these data demonstrate elevated expression levels of H19 in endometriosis patients promote abnormal glucose metabolism and elevated histone lactylation levels in vivo, enhancing cell proliferation and migration and promoting the progression of endometriosis. Our study provides a functional link between H19 expression and histone lactylation and glucose metabolism in endometriosis, providing new insights into disease mechanisms that could result in novel therapeutic approaches.

Open access