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Nan Meng NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

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Xinyue Wang Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China

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Yan Shi NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

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Yanyan Mao NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

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Qian Yang NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

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Baohui Ju Department of Gynecology and Obstetrics, The Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of China

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Qianxi Zhu NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

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Tingting Zhang Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China

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Yan Gu Department of Gynecology and Obstetrics, The Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of China

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Xuan Zhang NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of China

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Decidualization is essential for the successful establishment of pregnancy, and the dysregulated decidualization may lead to early pregnancy loss. It was previously reported by us that miR-3074-5p could promote apoptosis but inhibit invasion of human extravillous trophoblast (EVT) cells in vitro, and the expression level of miR-3074-5p in villus tissues of recurrent miscarriage (RM) patients was significantly increased. The aim of this study was to preliminarily explore the role of miR-3074-5p played in the decidualization of human endometrial stromal cells (ESCs). It was found that the decidual expression level of miR-3074-5p in RM patients was remarkably higher than that in the control group. The overexpression of miR-3074-5p in the immortalized human ESC line, T-HESCs, showed suppressive effects not only on the cell proliferation, as well as the intracellular expression levels of cyclin B1 (CCNB1), CCND1 and CCNE1 but also on the in vitro-induced decidualization. CLN8 mRNA, encoding an endoplasmic reticulum (ER)-associated membrane protein, was validated to be directly targeted by miR-3074-5p. And, the expression level of CLN8 was continuously increased along with the decidualization process, whereas down-regulated CLN8 expression could inhibit the decidualization of T-HESCs in vitro. Furthermore, contrary to the increased expression level of miR-3074-5p, a significantly decreased CLN8 expression was observed in decidual tissues of RM patients. Collectively, these data suggested that an increased miR-3074-5p expression in ESCs might cause early pregnancy failure by disturbing decidualization of ESCs via the miR-3074-5p/CLN8 pathway, providing a potential diagnostic and therapeutic target for RM.

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