Search Results

You are looking at 1 - 2 of 2 items for

  • Author: Zhi-Bin Li x
  • Refine by Access: All content x
Clear All Modify Search
Mei-rong Zhao State Key Laboratory of Reproductive Biology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Beijing 100080, People’s Republic of China and Graduate School of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China

Search for other papers by Mei-rong Zhao in
Google Scholar
PubMed
Close
,
Wei Qiu State Key Laboratory of Reproductive Biology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Beijing 100080, People’s Republic of China and Graduate School of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China

Search for other papers by Wei Qiu in
Google Scholar
PubMed
Close
,
Yu-xia Li State Key Laboratory of Reproductive Biology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Beijing 100080, People’s Republic of China and Graduate School of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China

Search for other papers by Yu-xia Li in
Google Scholar
PubMed
Close
,
Zhi-bin Zhang State Key Laboratory of Reproductive Biology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Beijing 100080, People’s Republic of China and Graduate School of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China

Search for other papers by Zhi-bin Zhang in
Google Scholar
PubMed
Close
,
Dong Li State Key Laboratory of Reproductive Biology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Beijing 100080, People’s Republic of China and Graduate School of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China

Search for other papers by Dong Li in
Google Scholar
PubMed
Close
, and
Yan-ling Wang State Key Laboratory of Reproductive Biology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Beijing 100080, People’s Republic of China and Graduate School of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China

Search for other papers by Yan-ling Wang in
Google Scholar
PubMed
Close

Transforming growth factor β (TGFβ) has been shown to be a multifunctional cytokine required for embryonic development and regulation of trophoblast cell behaviors. In the present study, a non-transformed cell-line representative of normal human trophoblast (NPC) was used to examine the effect of TGFβ1 on trophoblast cell adhesion and invasion. In vitro assay showed that TGFβ1 could significantly promote intercellular adhesion, while inhibiting cell invasion across the collagen I-coated filter. Reverse transcription (RT)-PCR and gelatin zymography demonstrated that TGFβ1 evidently repressed the mRNA expression and proenzyme production of matrix metalloproteinase (MMP)-9, but exerted no effect on mRNA expression and secretion of MMP-2. On the other hand, both the mRNA and protein expression of epithelial-cadherin and β-catenin were obviously upregulated by TGFβ1 in dose-dependent fashion, as revealed by RT-PCR and western-blot analysis. What is more, one of the critical TGFβ signaling molecules – Smad2 was notably phosphorylated in TGFβ1-treated NPC cells. The data indicates that cell invasion and adhesion are coordinated processes in human trophoblasts and that there exists paracrine regulation on adhesion molecules and invasion-associated enzymes in human placenta.

Free access
Hong-Jie Yuan Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Hong-Jie Yuan in
Google Scholar
PubMed
Close
,
Zhi-Bin Li Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Zhi-Bin Li in
Google Scholar
PubMed
Close
,
Xin-Yue Zhao Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Xin-Yue Zhao in
Google Scholar
PubMed
Close
,
Guang-Yi Sun Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Guang-Yi Sun in
Google Scholar
PubMed
Close
,
Guo-Liang Wang Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Guo-Liang Wang in
Google Scholar
PubMed
Close
,
Ying-Qi Zhao Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Ying-Qi Zhao in
Google Scholar
PubMed
Close
,
Min Zhang Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Min Zhang in
Google Scholar
PubMed
Close
, and
Jing-He Tan Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, People’s Republic of China

Search for other papers by Jing-He Tan in
Google Scholar
PubMed
Close

Mechanisms by which female stress and particularly glucocorticoids impair oocyte competence are largely unclear. Although one study demonstrated that glucocorticoids triggered apoptosis in ovarian cells and oocytes by activating the FasL/Fas system, other studies suggested that they might induce apoptosis through activating other signaling pathways as well. In this study, both in vivo and in vitro experiments were conducted to test the hypothesis that glucocorticoids might trigger apoptosis in oocytes and ovarian cells through activating the TNF-α system. The results showed that cortisol injection of female mice (1.) impaired oocyte developmental potential and mitochondrial membrane potential with increased oxidative stress; (2.) induced apoptosis in mural granulosa cells (MGCs) with increased oxidative stress in the ovary; and (3.) activated the TNF-α system in both ovaries and oocytes. Culture with corticosterone induced apoptosis and activated the TNF-α system in MGCs. Knockdown or knockout of TNF-α significantly ameliorated the pro-apoptotic effects of glucocorticoids on oocytes and MGCs. However, culture with corticosterone downregulated TNF-α expression significantly in oviductal epithelial cells. Together, the results demonstrated that glucocorticoids impaired oocyte competence and triggered apoptosis in ovarian cells through activating the TNF-α system and that the effect of glucocorticoids on TNF-α expression might vary between cell types.

Restricted access