APPLICATION OF A LIQUID-SCINTILLATION TECHNIQUE TO THE STUDY OF AEROBIC 14CO2 PRODUCTION FROM GLUCOSE AND FRUCTOSE BY BOAR, RAM AND BULL SPERMATOZOA

in Reproduction

Summary.

A technique which depends on absorbing 14CO2 on paper strips containing small amounts of KOH, and 'counting' the strips directly in a liquid-scintillation system, has been applied to studies on spermatozoal metabolism. A small quantity of uniformly-labelled [14C]-glucose or [14C]-fructose has been added to washed spermatozoal suspensions which already contained non-labelled glucose or fructose and the 14CO2 production assessed at the end of a 1 hr incubation period in an air atmosphere, at 38° C. Boar spermatozoa, like those of ram, bull and man, have been shown to utilize glucose preferentially if presented with a mixture of these two sugars.

If the inline PDF is not rendering correctly, you can download the PDF file here.

 

     An official journal of

    Society for Reproduction and Fertility

 

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 80 17 2
PDF Downloads 90 36 1