Regulation of steroid production in ovarian stromal tissue from 5- to 8-week-old pullets and laying hens

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Authors:
J. M. Levorse
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A. L. Johnson
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DOI:
https://doi.org/10.1530/jrf.0.1000195
Volume/Issue:
Volume 100: Issue 1
Page Range:
195–202
Article Type:
Research Article
Online Publication Date:
Jan 1994
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Studies were conducted to evaluate the regulation of steroid production in dispersed cells from ovarian stromal tissue from 5- to 8-week-old-pullets (IM cells) and laying hens (MAT cells). Short-term incubation of IM and MAT cells with ovine (o) LH resulted in a dose-dependent increase in progesterone, androstenedione and oestradiol production; progesterone production was greater in MAT cells than in IM cells (P < 0.05) in response to 2–200 ng oLH ml−1, whereas androstenedione and oestradiol production was greater in MAT cells following treatment with 20 and 200 ng oLH ml−1 (P < 0.05). In both cell populations the cyclic adenosine monophosphate (cAMP) analogue, 8-bromo-cAMP (1 and 10 mmol l−1) stimulated progesterone and androstenedione production, whereas oLH (200 ng ml−1) and forskolin (1–10 μmol l−1) promoted cAMP accumulation (P < 0.05 compared with basal values). However, treatment with the protein kinase C activator, phorbol 12-myristate 13-acetate (PMA), did not alter basal or oLH-stimulated cAMP accumulation or progesterone production in either IM or MAT cells (P > 0.10). PMA did, however, inhibit agonist-induced androstenedione production (P < 0.05); co-treatment with the calcium ionophore A23187 potentiated this inhibitory effect. Finally, treatment with transforming growth factor-α (TGF-α; 1.8–18 pmol l−1) did not affect basal or oLH-stimulated progesterone or androstenedione production by IM cells, MAT cells, theca cells from 6–8 mm follicles or theca cells from the second largest (F2) follicle (P > 0.10). We conclude that LH-stimulated steroid production is greater in MAT cells than in IM cells; production of steroids at both stages occurs, at least in part, via the adenylyl cyclase/cyclic AMP second messenger pathway. However, we propose that activation of protein kinase C can inhibit agonist-induced cytochrome P450 17α-hydroxylase, but not cytochrome P450 cholesterol side-chain cleavage, activity in stromal cells. Finally, steroidogenesis in stromal tissue from 5- to 8-week-old pullet and laying hen ovaries is regulated by hormonal and cellular mechanisms most comparable to those that modulate steroidogenesis in theca cells from 6- to 8-mm and F2 follicles.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899