Effect of immunization of rams against bovine inhibin α1–26 on semen characteristics, scrotal size, FSH, LH and testosterone concentrations

in Reproduction
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R. M. McKeown
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D. O'Callaghan
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J. F. Roche
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M. P. Boland
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The effects of inhibin immunization on inhibin antibody titres, semen characteristics, scrotal size, fertility, FSH, LH and testosterone concentrations were determined by immunizing adult rams against bovine inhibin α1–26-Gly-Tyr conjugated to human serum albumin (n = 16) in non-ulcerative Freund's adjuvant and DEAE:dextran (1:1) or adjuvant alone (n = 16) on days 0 (29 June), 30, 60, 191, 303 and 394. Blood samples were collected and bovine inhibin α1–26-Gly-Tyr antibody titres and serum testosterone concentrations were determined. Each month, between days 174 and 417, semen was collected every 30 min to a maximum of 15 ejaculates over 7 h and scrotal circumference was measured. Ram fertility was recorded during natural service. FSH, LH and testosterone concentrations and GnRH-induced FSH and LH release were measured in a subgroup of immunized (n = 5) and control (n = 5) rams at frequent intervals. Antibody titres were variable among immunized rams (0–46% I-labelled bovine inhibin α1–26-Gly-Tyr at 1:1600 serum dilution) but mean titres were consistently higher than in control rams (P ≤ 0.001). Immunization did not alter the semen volume, output or quality of spermatozoa or ram fertility, but increased the mean scrotal circumference (37.6 ±0.8 cm versus 34.4 ± 0.7 cm, P < 0.001). Mean FSH concentrations were higher in immunized rams during two intensive blood sampling periods (in June and August) (5.8 ± 0.7 ng ml−1 versus 3.0 ± 0.3 ng ml−1, P < 0.001 in June; and 4.8 ± 0.9 ng ml−1 versus 2.0 ± 0.3 ng ml−1, P < 0.02 in August), and were correlated with antibody titres (r2 = 0.3, P < 0.05 in June; and r2 = 0.8, P < 0.001 in August). Discrete FSH pulses were not detected. Immunization did not alter mean or basal testosterone or LH concentrations, or LH pulse frequency; LH pulse amplitude was increased (1.6 ± 0.2 ng ml−1 versus 0.8 ± 0.2 ng ml−1, P < 0.02) and was correlated with antibody titres (r2=0.6, P < 0.01). Immunization enhanced GnRH-induced FSH (P <0.05) but not LH release. In conclusion, immunization of adult rams against bovine inhibin α1–26 Gly-Tyr increased scrotal circumference, mean FSH concentrations and LH pulse amplitude, without altering semen characteristics, fertility, mean LH concentrations, LH pulse frequency or mean testosterone concentrations.

 

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