In order to study the effect of irradiation on testicular function it is important to know the frequency with which ejaculates must be taken to establish accurately the post-irradiation pattern of sperm output. The interval from irradiation to the onset of sterility in mice is said to be unaffected by varying the frequency of mating (Bateman, 1958). 32Phosphorus-labelled spermatozoa appear in the ejaculate of bulls at approximately the same time whether they are collected two to three times a day or once a week (Koefoed-Johnsen, 1960). On the other hand, Cox & Willham (1961) found that irradiated boars reached their minimum sperm output some 12 days later when collected once a week than when collected three times a week, although the pattern of recovery in sperm production was not altered.
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