Despite repeated attempts by many workers to transfer eggs to the cow by non-surgical methods, no success was achieved until the recent report of a single pregnancy by Mutter, Graden & Olds (1964). Subsequently, Sugie (1965) using a technique involving the puncture of the anterior wall of the vagina and the inflation of the uterus with carbon dioxide reported two more successful transfers; one of which however was followed by abortion.
The difficulty inherent in the technique of non-surgical transfer of cow eggs was attributed by Rowson, Lamming & Fry (1953), Harper, Bennett & Rowson (1961) and Bennett & Rowson (1961) to at least two causes (i) uterine infection, and (ii) expulsion of the eggs via the cervix, the two factors acting either singly or in conjunction with each other. Our efforts to overcome these
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