During the course of some behavioural studies of the Golden Hamster, pseudopregnancy has been induced by copulation with sterile males or the injection of various gestagens. The duration of pseudopregnancy was found to be significantly extended in some of the groups receiving gestagen injection.
Virgin females, 2 to 3 months old, were housed individually in standard M.R.C. rat cages. They were kept on a 12/12 hr reversed light schedule and given unlimited diet 41B and water. Each morning, in the hour preceding the dark half of the light schedule, vaginal smears were taken and read by the method of Orsini (1961) which is based on colour and viscosity of the vaginal mucus. Pseudopregnancy was inferred from the continued presence of a clear, highly viscous secretion; it was not confirmed by laparotomy. All injections and sterile matings (using vasectomized
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