Man's control of the sex ratio of progeny remains one of the most interesting unsolved problems in biology. Many experiments have been done to separate the male-determining Y-spermatozoa and the female-determining X-spermatozoa. Up to the present time, no method to influence sex ratio in mammals has been successfully developed. After the war, the theory of size-differences between X-and Y-spermatozoa was again tested. Lindahl (1958) carried out experiments with counter-streaming centrifugation of bull spermatozoa. Inseminations with heavy spermatozoa produced more female calves (57%), with light spermatozoa more male calves (59%).
In order to separate X- and Y-sperm-cells by their different size and weight, Bhattacharya (1958, 1962) made use of the spermatozoa's own gravity dropping through a medium of high viscosity. He took advantage of an instrumental principle developed by Kampschmidt, Mayer, Hermann &
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