The Michaelis-Menten constant (KM) of fructose and oi glucose as substrate for bull spermatozoa has been measured: fructose KM = 1·04 mM, glucose KM = 0·06 mM. The saturation rate of fructose utilization was found to be 1·8 times that of glucose utilization. This is in general agreement with characteristics of hexokinases extracted from different sources.
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