ATTEMPTS TO DEMONSTRATE 3β- AND 17β-HYDROXY-STEROID DEHYDROGENASES HISTOCHEMICALLY IN THE TESTES OF THE STALLION, BOAR, RAM AND BULL

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Authors:
MARY F. HAY
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HELEN WENDLER DEANE
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DOI:
https://doi.org/10.1530/jrf.0.0120551
Volume/Issue:
Volume 12: Issue 3
Page Range:
551–560
Article Type:
Research Article
Online Publication Date:
Dec 1966
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Summary.

An attempt has been made to study the distribution of Δ5-3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase and diaphorase activities in the testes of mature stallions, boars, rams and bulls and immature piglets and calves.

Using the standard histochemical medium, Δ5-3β-hydroxysteroid dehydrogenase activity was found only in the Leydig cells of the horse, and in a few interstitial cells of some rams. A false positive reaction ('nothing dehydrogenase') was obtained in porcine testes, and no modification of the method could eliminate this. Lowering of the concentration of co-enzyme in the medium was necessary to bring out selective staining of the interstitial tissue in bovine testes.

17β-Hydroxysteroid dehydrogenase activity could not be demonstrated in the testes of any of the species examined when testosterone was used as a substrate.

Enzymes that oxidize the reduced co-enzymes, nadh2 and nadph2 (diaphorases), were demonstrable in testes of all four species in both the seminiferous tubules and interstitial cells.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899