FIXATION OF CARBON DIOXIDE BY RAM SPERMATOZOA

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Authors:
T. O'SHEA
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and
R. G. WALES
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DOI:
https://doi.org/10.1530/jrf.0.0140333
Volume/Issue:
Volume 14: Issue 2
Page Range:
333–334
Article Type:
Research Article
Online Publication Date:
Oct 1967
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As the effect of carbon dioxide on mammalian spermatozoa is of current interest (Lodge & Salisbury, 1963; Hammer & Williams, 1964; VanDemark, Koyama & Lodge, 1965; Wales & O'Shea, 1966; Wales & Restall, 1966), the possibility of its fixation by ram spermatozoa has been investigated.

Aliquots (0·4 ml) of ram semen or twice-washed spermatozoa (5·4 × 108 cells/ flask) were incubated at 37° C with [14C]sodium bicarbonate (1 μmole and 25 μc) in phosphate-buffered saline containing 1 mm-potassium chloride, 2mm-magnesium chloride, 10 mm-sodium Pyruvate, 30 mg/100 ml penicillin and 50 mg/100 ml streptomycin. After 15 or 60 min, 0·5 ml of the incubated semen was removed and stored at -15° C for subsequent chromatography. The remaining 0·5 ml was acidified (0·1 ml of 6 n-H2

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Print ISSN:
1470-1626
Online ISSN:
1741-7899