The fertilization of rat eggs after various treatments of, or the injection of various substances into, the Fallopian tubes just before fertilization was studied. After all treatments, an increase was observed in the number of eggs which contained supplementary spermatozoa and in the numbers of supplementary spermatozoa per egg. Most treatments produced a small number of eggs in which a second spermatozoon entered the vitellus later than the first, and these eggs were said to be `refertilized'. Several other abnormalities were produced by the treatments.
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