Repeated superovulation and in-vivo collection of ova were carried out with thirty does divided into three groups of ten does each. One group received fsh—lh, a second group received pmsg—hcg and the controls received only lh. Injections of fsh—lh at three 16-week intervals followed by one 8-week interval resulted in 46·5, 35·4, 25·2 and 18·0 ovulation points/doe. This decrease with time was significant (P<0·05). Corresponding values for the pmsg—hcg group which, however, received fsh—lh for the final superovulation were 13·6, 5·7, 6·2 and 20·3. The lh controls averaged 7·8, 7·0, 5·1 and 5·6 ovulation points. Overall treatment differences were highly significant (P<0·005). From the 1920 ovulation points 1593 ova (83%) were recovered, of which 83·1% were cleaved. Young born from unrecovered ova accounted for 3·1%.
Control kindlings by the same does at regular periods resulted in normal litter size but in fewer does kindling as the experiment progressed. Results of two bio-assays for antihormones suggested that this decrease was due to hormonal refractoriness which was most pronounced in the pmsg—hcg group.
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