The colloid mill was found capable of disrupting bovine epididymal spermatozoa in such a way that even the very resistant sperm middle piece was opened. The homogenate thus produced was fractionated by both differential centrifugation and isopycnic density gradient centrifugation. The latter method was found very useful in producing homogenous fractions of cell components including in order of increasing density : mitochondrial fragments, acrosomes, membranes, tail principal pieces, middle pieces, middle piece exteriors, contractile fibres and denuded heads. The mitochondrial particles consumed oxygen while the cell membranes had hexokinase-like activity.
If the inline PDF is not rendering correctly, you can download the PDF file here.