A DISRUPTION AND FRACTIONATION OF BOVINE EPIDIDYMAL SPERMATOZOA

in Reproduction

Summary.

The colloid mill was found capable of disrupting bovine epididymal spermatozoa in such a way that even the very resistant sperm middle piece was opened. The homogenate thus produced was fractionated by both differential centrifugation and isopycnic density gradient centrifugation. The latter method was found very useful in producing homogenous fractions of cell components including in order of increasing density : mitochondrial fragments, acrosomes, membranes, tail principal pieces, middle pieces, middle piece exteriors, contractile fibres and denuded heads. The mitochondrial particles consumed oxygen while the cell membranes had hexokinase-like activity.

If the inline PDF is not rendering correctly, you can download the PDF file here.

 

     An official journal of

    Society for Reproduction and Fertility

 

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 65 14 0
PDF Downloads 206 45 2