The ovarian ascorbic acid depletion (oaad) assay of Parlow (1958) is an established method for the measurement of luteinizing hormone (lh). Tests using purified preparations of other anterior pituitary hormones have shown the bio-assay to be specific for lh (McCann & Taleisnik, 1960; Schmidt-Elmendorff & Loraine, 1962) but vasopressin possesses appreciable activity (McCann & Taleisnik, 1960). Pelletier (1964) has reported that hypophysectomized ewe plasma caused a significant response when assayed by this method. Evidence has been produced suggesting that the plasma acted by releasing lh from the pituitary glands of the assay rats (Pelletier, 1965).
Recently, determinations of the pituitary content of lh in the sow at various reproductive stages and after ovariectomy have been carried out in this laboratory. A preliminary report of this work has
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