Genetics of male infertility: from research to clinic

in Reproduction

Male infertility is a multifactorial complex disease with highly heterogeneous phenotypic representation and in at least 15% of cases, this condition is related to known genetic disorders, including both chromosomal and single-gene alterations. In about 40% of primary testicular failure, the etiology remains unknown and a portion of them is likely to be caused by not yet identified genetic anomalies. During the last 10 years, the search for ‘hidden’ genetic factors was largely unsuccessful in identifying recurrent genetic factors with potential clinical application. The armamentarium of diagnostic tests has been implemented only by the screening for Y chromosome-linked gr/gr deletion in those populations for which consistent data with risk estimate are available. On the other hand, it is clearly demonstrated by both single nucleotide polymorphisms and comparative genomic hybridization arrays, that there is a rare variant burden (especially relevant concerning deletions) in men with impaired spermatogenesis. In the era of next generation sequencing (NGS), we expect to expand our diagnostic skills, since mutations in several hundred genes can potentially lead to infertility and each of them is likely responsible for only a small fraction of cases. In this regard, system biology, which allows revealing possible gene interactions and common biological pathways, will provide an informative tool for NGS data interpretation. Although these novel approaches will certainly help in discovering ‘hidden’ genetic factors, a more comprehensive picture of the etiopathogenesis of idiopathic male infertility will only be achieved by a parallel investigation of the complex world of gene environmental interaction and epigenetics.


Male infertility is a multifactorial complex disease with highly heterogeneous phenotypic representation and in at least 15% of cases, this condition is related to known genetic disorders, including both chromosomal and single-gene alterations. In about 40% of primary testicular failure, the etiology remains unknown and a portion of them is likely to be caused by not yet identified genetic anomalies. During the last 10 years, the search for ‘hidden’ genetic factors was largely unsuccessful in identifying recurrent genetic factors with potential clinical application. The armamentarium of diagnostic tests has been implemented only by the screening for Y chromosome-linked gr/gr deletion in those populations for which consistent data with risk estimate are available. On the other hand, it is clearly demonstrated by both single nucleotide polymorphisms and comparative genomic hybridization arrays, that there is a rare variant burden (especially relevant concerning deletions) in men with impaired spermatogenesis. In the era of next generation sequencing (NGS), we expect to expand our diagnostic skills, since mutations in several hundred genes can potentially lead to infertility and each of them is likely responsible for only a small fraction of cases. In this regard, system biology, which allows revealing possible gene interactions and common biological pathways, will provide an informative tool for NGS data interpretation. Although these novel approaches will certainly help in discovering ‘hidden’ genetic factors, a more comprehensive picture of the etiopathogenesis of idiopathic male infertility will only be achieved by a parallel investigation of the complex world of gene environmental interaction and epigenetics.


Nearly 7% of men from the general population are infertile and in at least 15% of cases this condition is related to genetic disorders, including both chromosomal and single-gene alterations. Genetic causes can be detected in all major etiologic categories of male infertility (pre-testicular, testicular and post-testicular forms) and genetic tests became part of the routine diagnostic procedure in selected groups of patients (Krausz 2011). Karyotype and azospermia factor (AZF) microdeletion analyses are indicated in patients with <10 million spermatozoa/ml and <5 million spermatozoa/ml respectively (Krausz et al. 2014). CFTR gene mutation screening is performed in men affected by congenital absence of vas deferens, whereas in the case of central hypogonadism a growing number of candidate genes involved in gonadotrophin-releasing hormone receptor migration, development, secretion and response can be analyzed. After a complete diagnostic work-up (including also genetic testing), in about 40% of primary testicular failure the etiology remains unknown and is referred to as ‘idiopathic infertility.’ The search for ‘hidden’ genetic factors, especially focusing on polymorphisms, in idiopathic infertile patients were intensified in the late 1990s, since this approach turned out to be successful in some other complex multifactorial diseases (Riggs et al. 2014, Smith & Newton-Cheh 2015). Starting from 2009, novel approaches such as single nucleotide polymorphism (SNP) array, comparative genomic hybridization-array (array-CGH) and next generation sequencing (NGS) provided important data also on rare variants. This review is aimed at providing an overview of i) genetic risk factors including SNPs, variable number tandem repeats (VNTRs) and copy number variations (CNVs) and ii) potential causative mutations/CNVs related to idiopathic male infertility.

Genetic susceptibility factors: the candidate gene approach

Since late 1990s, the field of genetics of male infertility entered an era of intense search for genetic risk factors, mainly SNPs, VNTRs and Y chromosome-linked CNVs. The results obtained up to 2007 have been summarized in the meta-analysis by Tüttelmann et al. (2007), who reported significant association with impaired spermatogenesis only for two genetic factors: a partial AZFc deletion (gr/gr deletion) and the rs1801133 (c.677C>T) variant in the MTHFR gene. At that time, for many other SNPs, either only single studies were available or results from different laboratories were discordant (Nuti & Krausz 2008).

We herein review the existing literature via a search in the PubMed database of case–control studies published since 2008. The following keywords were used to select eligible studies: ‘genetic risk factor (s)’ AND ‘male infertility.’ Additionally, all identified gene/polymorphism combinations were searched individually (e.g., ‘FASLG’ and ‘male (in)fertility’). Data were extracted from single papers and are summarized in Tables 1, 2 and 3 and Supplementary Table 1.

Table 1

Summary of case-control studies focusing on gene polymorphisms since 2008. SNPs related to genes with (A) common cell function, (B) specific spermatogenic function, (C) endocrine function. Further details are given in the Supplementary Table 1, see section on supplementary data given at the end of this article.

Gene nameCases+controlsCountry of originAssociation
(A) Common cell function
AHR991+1256China; Estonia; Iran; JapanYES**
AHRR235+324Estonia; JapanDISCORDANT
APOB604+501Slovenia; IndiaDISCORDANT
ARNTLa589+444Slovenia, SerbiaNO
CAT885+839China; France; IranDISCORDANT
ETV5a204+296Australia, USAYES
FAS547+571China; India; TurkeyNO
FASLG447+532Albania, Macedonia; China; TurkeyNO
GPX1690+649China; FranceNO
HLA-DRA4508+7588China; JapanYES
JMJDIAa136+161Albania, MacedoniaNO
LOC203413623+530Albania, Macedonia; JapanNO
LRWD1 130+100JapanNO
MCT2 (SLC16A7)a471+265KoreaYES
MTHFD1428+533Sweden; RussiaNO
MTR713+739Brazil; China; PolandNO
MTRR1790+1622Brazil; China; France; Jordania; Korea; Poland; SwedenDISCORDANT
NFE2L2 (NRF2)a336+295ChinaYES
OR2W3623+530Albania, Macedonia; JapanDISCORDANT
PEX102369+2946China; JapanNO
PON11037+1094China; Greece; Iran; SloveniaDISCORDANT
PON2270+320Greece; IranDISCORDANT
SOD2690+649China; FranceDISCORDANT
SOX52987+3526China; JapanDISCORDANT
TAS2R38623+530Macedonia, Albania and JapanNO
(B) Specific spermatogenic function
 BRDT259+343Albania, Macedonia; IsraelNO
 H2BFWT851+445China; KoreaYES
 HORMAD1391+448China; JapanYES**
 PRM1851+955China; Iran; Japan; SpainYES**
 PRM2525+648China; JapanNO
 PRMT62369+2946China; JapanNO
 SEPT12290+480Japan; TaiwanDISCORDANT
 SPO11186+167China; IranDISCORDANT
 TEX15445+538Albania, Macedonia; ChinaNO
 UBE2B568+612China and IndiaYES*a
(C) Endocrine function
 INSR624+530Albania, Macedonia; JapanNO

Underlined, gene polymorphisms evaluated in meta-analyses comprising study populations with different ethnic/geographic origins and association description refers to the global meta-analysis results; YES, SNP is associated in all studies; YES**, multiple SNPs studied in the gene by different authors, but specific SNPs analyzed in a single study result as associated to male infertility; DISCORDANT, the same SNP analyzed in different studies show discordant results; NO, SNP shows no association in any study.

Gene analyzed by a single study. Alternative gene names appearing in other studies are reported in brackets.

Table 2

Summary of GWAS results. SNPs and related genes described as significantly associated in GWA Studies.

Aston & Carrell (2009)Aston et al. (2010)aHu et al. (2012)Zhao et al. (2012)Kosova et al. (2012)
SNP associatedGene relatedSNP associatedGene relatedSNP associatedGene relatedSNP associatedGene relatedSNP associatedGene related

Aston et al. (2010) analyzed a total of 172 SNPs including also 84 SNPs from Aston & Carrell (2009).

Table 3

Summary of GWAS replication studies for SNPs and related genes (including SNPs presenting significant or borderline association in the original GWAS).

ReferenceSNPs analyzedGene related
Follow-up Aston et al. (2010)
 Plaseski et al. (2012)ars5911500bLOC203413
 Chihara et al. (2015)rs11204546bOR2W3
Follow-up Hu et al. (2012)
 Xu et al. (2013)rs3197744bSIRPA
 Lu et al. (2014)rs1048055bSIRPG
 Zou et al. (2014)rs10842262bSOX5
 Hu et al. (2014)crs7194bHLA-DRA
 Sato et al. (2013)rs12097821PRMT6
Follow-up Hu et al. (2012), Zhao et al. (2012)
 Tu et al. (2014)rs3129878bHLA-DRA
Follow-up Zhao et al. (2012)
 Jinam et al. (2013)rs3129878bHLA-DRA
Follow-up Kosova et al. (2012)
 Sato et al. (2015)rs7867029bPSAT1

SNPs in this study are not significantly associated after Bonferroni correction.

SNPs described as significantly associated.

Only SNPs described as significantly associated to male infertility are listed (in the study, a total of 77 SNPs originated from the Hu et al. (2012) paper were screened).

As in other fields of medicine, targeted search for SNPs or gene mutations is based on the candidate gene approach. This approach has been facilitated by an increasing body of information from model organisms, expression analyses (transcriptomic and proteomic) in relationship with spermatogenesis and, together with data produced by Genome-Wide Association Studies (GWAS) (Tables 2 and 3), represents the major source for genetic studies in humans. A minority of SNPs (n=28) studied before 2008 have been the objects of subsequent publications, whereas the large majority, listed in Table 1, are new entries (n=286). A total of 314 SNPs have been reported in 123 genes. Approximately 70% of SNPs are related to genes with common cell function but with predicted relevance in germ cells, such as apoptotic process, DNA repair, detoxification of environmental molecules, response to reactive oxygen species and so on. Indeed, the best candidate genes are those with specific expression in germ cells or those that have specific spermatogenic function or play important roles in meiosis or endocrine regulation of the testis (Table 1). Data in existing literature are rarely concordant, and for many SNPs (n=269), only single studies are available. To date, meta-analyses are available for ten genes: AR, CYP1A1, DAZL, ESR1, ESR2, MTHFR, NOS3, POLG, TP53 and USP26. Although data remains largely controversial, ethnic/geographic origin seems to play an important role in the phenotypic expression of polymorphisms in the MTHFR, ESR1/ESR2, NOS3 and DAZL. Data remains inconclusive for CYP1A1 and AR genes, whereas a lack of association with male infertility has been clearly demonstrated for polymorphisms related to TP53, USP26 and POLG. Although reliability of the presently available meta-analyses is largely limited by the heterogeneous inclusion criteria used for patients and controls selection, in this review we attempt to provide a short description of those SNPs that according to the latest meta-analyses result significantly associated with spermatogenic failure.

Tüttelmann et al. (2007) reported that the c.677C>T variant in the MTHFR (methylenetetrahydrofolate reductase (NAD(P)H) gene was the only one showing significant association with male infertility. The MTHFR gene is located on chromosome 1p.36.22, encodes an enzyme that produces 5-methyltetrahydrofolate and is in involved in folate metabolism. Folate is necessary for the preservation of genome integrity due to its role in DNA synthesis, repair and methylation, and it has been predicted that its deficiency may lead also to male infertility. The c.677C>T variant impairs the enzyme activity by 35% in heterozygosis and by 70% in homozygosis (Frosst et al. 1995). The conclusion presented by Tüttelmann et al. (2007) stimulated further studies, which led to controversial results and to novel meta-analyses (Gupta et al. 2011, Wei et al. 2012, Wu et al. 2012, Weiner et al. 2014, Gong et al. 2015). Interestingly, there is discordance even between the five meta-analyses, with some reporting an association (Tüttelmann et al. 2007, Gupta et al. 2011, Wu et al. 2012) and others reporting a lack of association (Wei et al. 2012, Weiner et al. 2014). The last meta-analysis (Gong et al. 2015), which included 26 published studies (5575 cases and 5447 controls from Asian, African and Caucasian populations), indicated that the MTFHR variant is associated with AZ (AZ) (OR=1.36, 95% CI: 1.18–1.55, P=0.000) and oligoasthenoteratozoospermia (OAT) (OR=1.35, 95% CI: 1.11–1.64, P=0.003), but not with oligozoospermia. Finally, a second SNP in the MTHFR gene has also been the object of numerous studies but with similar discordant results. Rs1801131, also known as 1298C>A, is a missense polymorphism found in exon 7 that also reduces MTHFR activity, though apparently less severely than C677T (Van der Put et al. 1998). The meta-analysis of seven studies with a total of 1633 cases and 1735 controls from different ethnic groups shows that the polymorphism is significantly associated with azoospermia (OR=1.12, 95% CI=1.00–1.26) but not with OAT (Shen et al. 2012).

Overall, for both SNPs the conferred susceptibility to AZ and OAT is modest, implying a marginal biological role for this SNP in infertility. Controversies might depend on different ethnic origin (variant frequency does differ among different populations), and the penetrance of this mutation is likely to be affected by diet, e.g., subjects carrying the variant may have a major risk for male infertility in cases of low folate intake. Consequently, it could be of interest to test for these SNPs in relationship to the responsiveness to folate supplementation, i.e., to select potential ‘responders’ through a pharmacogenetic approach.

Other SNPs that have been objects of investigation occur in the estrogen receptor 1 (ESR1) and estrogen receptor 2 (ESR2) genes. Estrogens are predicted to play an important role in the male reproductive tract, and both the deficit and the excess of estrogens can alter sperm production and maturation (Atanassova et al. 1999, Hess 2003). Three different receptor isoforms ERα, and ERγ are known. The ESR1 gene on 6q25 codifies for ERα, a 595 amino acid receptor. The ESR2 gene is located on chromosome 14q23-24 and codifies for ERβ, a protein with 530 amino acids. Both receptors are highly expressed in human testicular germ cells. Regarding ESR1, the two most studied SNPs are rs2234693 (also known as PvuII) and rs9340799 (known as XbaI), both located in intron 1 (c.453-397T>C and c.453-351A>G respectively). Although a relationship between these SNPs and ESRs gene/protein function and stability has been proposed, their exact effect remains unclear. The last meta-analysis performed so far involves 12 studies comprising from 736 to 1418 infertile cases and 841–1601 controls depending on the type of analyzed SNP (Ge et al. 2014). The meta-analysis includes azoospermic, oligozoospermic and oligoasthenozoospermic (OAZ) and OAT patients of different ethnic and geographic origin. According to this analysis, ethnic background plays an important role in the biological effect of the variants. For instance, the minor allele C of rs2234693 (c.453-397T>C) seems to show a protective effect in the Asian population (C allele vs T allele OR=0.78, 95% CI: 0.64–0.96; CC vs TT, OR=0.61, 95% CI: 0.40–0.93), whereas in Caucasians it is associated with an increased risk for infertility (CC vs CT+TT: OR=1.52, 95% CI: 1.05–2.22). As far as the XbaI SNP (c.453-351A>G), the G allele is associated with a decreased risk, according to the dominant model in the Asian population, whereas no association was found in Caucasians. A similar situation was encountered also for the SNP rs1256049 in ESR2 (c.984G>A), which according to the recessive model is associated with a decreased risk in Asian populations, whereas in Caucasian men it is associated with an increased risk for male infertility according to the dominant model. Finally, rs4986938 (c.1406+1872G>A) mapped on ESR2 does not affect male fertility in any population. These results show again the importance of the patients’ ethnic origin and their genetic background in modulating the effect of a given variant. Controversies may also derive from the different level of exposition to endocrine disrupters, which also interact with these receptors and alter testis development and function. It is therefore plausible that a more pronounced effect of these SNPs can be observed only in relationship with a high level of exposure to these environmental factors.

As for the nitric oxide synthase 3 (NOS3 or eNOS) gene, three principal SNPs have been studied in relationship with male infertility: rs1799983 (c.894T>G in the exon 8), rs2070744 (c.-786C>T in the promoter region) and rs61722009 (27 bp VNTR polymorphisms in the intron 4, also known as 4a4b polymorphisms). NOS3 is located on chromosome 7q36.1 and produces nitric oxide (NO), which is implicated in several cellular functions such as vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway, but also predicted to have an important role in fertility, including sperm motility and maturation, as well as germ cell apoptosis in the testis (Zini et al. 1996, Lee & Cheng 2008). The eNOS rs2070744 variant is associated with reduced promoter activity, suppressed eNOS transcription and decreased NO generation (Dosenko et al. 2006). There is also a trend for diminished eNOS enzyme activity in eNOS rs1799983 SNP carriers (Wang &.Mahaney 1997). The VNTR within intron 4 of the eNOS gene accounts for >25% of basal plasma NO generation, suggesting that this gene might have an important role in NO-mediated physiology (Wang et al. 1997). The first case–control study related to fertility analyzed the three SNPs in a cohort of 371 patients and association was found only between the 4a4b variant and sperm morphology (Yun et al. 2008). Subsequently, relatively small studies from Italy, China, Iran and Brazil reached discordant results (Buldreghini et al. 2010, Safarinejad & Shafiei 2010, Bianco et al. 2013, Yan et al. 2014). Finally, Song et al. (2015) performed a meta-analysis on 2018 infertile patients (from eight studies, including their own) and concluded that only c.-786C>T and 4a4b were significantly associated with male infertility in both the Asian and Caucasian populations (OR=1.53, 95% CI=1.10–2.22 and OR=3.24, 95% CI=2.49–4.22 respectively). Indeed, these SNPs are promising and merit further investigations in order to define their potential clinical relevance.

The deleted in azoospermia-like (DAZL ) gene is an autosomal homologue of the Y-chromosomal DAZ (deleted in azoospermia) gene cluster and maps to chromosome 3p24 (Yen et al. 1996). As the other family members (DAZ and BOLL), this gene encodes RNA binding proteins with important roles in spermatogenesis (Yen 2004). One of the most studied SNPs is rs121918346, a missense variant that changes threonine 54 to an alanine on exon 3. The last meta-analysis comprised 13 studies with a total of 2715 cases and 1835 controls from different ethnic origins and concluded that the variant was significantly associated with male infertility exclusively in Chinese men (Chen et al. 2015). This finding is in line with the conclusion of the first Caucasian study that considered this polymorphism as ‘an example of remarkable ethnic differences’ for its effect on predisposing carriers to spermatogenic failure (Becherini et al. 2004).

The androgen receptor (AR) gene also contains two polymorphic sites in the N-terminal trans-activation domain of the receptor: a polyglutamine tract – (CAG)n – and a polyglycine tract – (GGC)n,, which were objects of many publications related to male infertility (for review see Davis-Dao et al. (2007) and Nenonen et al. (2011)) The (CAG)n length normally ranges between six and 39 repeats in the general population, with a median value that varies according to the ethnicity (21–22 in White Caucasian, 19–20 in African–American, 22–23 in Asian, 23 in Hispanic populations). The originally described inverse relationship between CAG repeat length and the receptor trans-activation led to the hypothesis that longer CAG repeat conferred a higher risk for a series of androgen-dependent diseases, including infertility and cryptorchidism (Tut et al. 1997). The first meta-analysis based on 33 publications in 2007 was unable to find a cut-off value above which infertility risk is increased (Davis-Dao et al. 2007). A more recent meta-analysis has proposed an alternative way of analysis based on the ‘optimal range’ hypothesis, which derives from novel functional studies reporting that the AR activity was actually higher in the presence of a determined number of CAG (Nenonen et al. 2011). Therefore, according to this hypothesis either a longer or a shorter CAG tract might have a negative effect on the receptor function. Although Nenonen et al. (2011) were able to demonstrate a significant association between the length of this polymorphism below or above the ‘optimal range’ and impaired sperm production (CAG <22: P=0.03, OR=1.18 95% CI: 1.02–1.39; for CAG >23: P=0.02, OR=1.22, 95% CI 1.03–1.44), the role of CAG repeats in male infertility is probably more complex than it has been previously considered. More functional and clinical studies are needed before the introduction of this polymorphism into the diagnostic setting.

The CYP1A1 (cytochrome P450, family 1, subfamily A, polypeptide 1) is located on chromosome 15q24.1 and encodes a member of the cytochrome P450 superfamily. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP1A1 encodes a 522-aminoacide protein that, among its functions, is involved in the metabolism of polycyclic aromatic hydrocarbons into their biologically active intermediates that have potential reproductive toxicity in men (McManus et al. 1990). The rs4646903 variant, a T>C substitution in 3’UTR of CYP1A1 gene has been associated with increased transcript half-life and therefore increased enzyme activity resulting in elevated levels of activated metabolites (Manfredi et al. 2007). This SNP has been associated with different types of cancers (Salnikova et al. 2013, Abbas et al. 2014), further supporting their biological importance. Studies focusing on the role of this SNP in male infertility overall produced discordant results even in the same ethnic groups. Despite discrepancies, the last meta-analysis performed on a total of 1060 cases and 1225 controls concluded for a significant association between the variant and male infertility reaching the highest risk's entity according to the homozygous model (OR=2.18, 95% CI: 1.15–4.12) (Luo et al. 2014). However, since only two out of six studies report it as a significant susceptibility factor, this meta-analysis awaits further confirmation. Given the biological function of this gene, differences in exposure to environmental factors may also influence the outcome of single studies; lack of information about careful matching of important variables such as drug and alcohol intake and life-style factors between patients and controls may well be responsible for controversies.

Apart from the meta-analyses focusing on the ten genes, in case of multiple studies analyzing the same SNPs/gene, results are almost constantly controversial and even if association is found generically with ‘infertility,’ the subgroup analysis shows differences (Supplementary Table 1). An example is the rs7885967 (c.-9C>T) of the H2BFWT (H2B histone family, member W, testis-specific) gene encoding for a testis-specific histone with an essential role during meiotic chromatin reorganization (Gineitis et al. 2001). This SNP maps to the 5’UTR of H2BFWT and has been demonstrated to affect the translation of the protein (Lee et al. 2009). The two case–control studies found significant association (with moderate OR ranging from 1.51–1.88) with completely different semen phenotypes: azoospermia in the Chinese population (Ying & Scott 2012) whereas lack of association with azoospermia and association with non-azoospermia (a heterogenous group of oligo/astheno/teratozoospermic men) in the Korean study (Lee et al. 2009). Such contradictory results clearly discourage further studies on this SNP.

The unique example of a polymorphism with fully concordant results in more than one relatively large independent study populations is related to the MSH5 gene (rs2075789). The mutS homolog 5 (MSH5) encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and apoptosis. Msh5 knockout mice present sterility due to the defect in resolving meiotic chromosomal crossovers (Edelmann et al. 1999) Yeast two-hybrid analysis demonstrated that the SNP rs2075789 impairs interaction between MSH4 and MSH5 proposing a functional effect (Yi et al. 2005). The two independent studies that include a total of 1454 cases and 640 controls from the Chinese population report a similar risk's entity for homo/heterozygous minor allele carriers compared to WT homozygous carriers (OR=2.51; 95% CI=1.43–4.40 and OR =1.83, 95% CI=1.32–2.55, by Xu et al. (2010) and Ji et al. (2012) respectively). Although this is a promising candidate SNP, its importance remains limited until new data are available in other populations.

Genetic susceptibility factors: GWAS and SNPs

All the genetic risk factors discussed above originate from the candidate gene approach, which is based on the analysis of genes/polymorphisms with predicted or known function in spermatogenesis. Given the relatively poor outcome of these studies, much expectation was given to whole genome analysis. Gene discoveries from GWAS have been successful for several diseases and helped unravel pathways important for a certain biological process (Visscher et al. 2012) Overall, four GWAS based on SNP-arrays are available in the literature and are summarized in Table 2 (Aston & Carrell 2009, Hu et al. 2012, Kosova et al. 2012, Zhao et al. 2012). The first study by Aston and Carrell (2009) analyzed 370 000 SNPs in 92 oligozoospermic and non-obstructive azoospermic (NOA) patients and 80 healthy controls and found 21 SNPs associated with azoospermia or oligozoospermia. Due to the prohibitively high cost of the array studies in 2009, the study population size was clearly underpowered and the associations reported did not reach genome-wide significance. This pioneer work was followed by two large, properly powered Chinese GWAS, which reported a number of SNPs with stringent P value <1×10−8. Hu et al. (2012) analyzed 2927 individuals with NOA and 5734 controls from Han Chinese population and found a few SNPs predisposing to NOA in PRMT6, PEX10 and SOX5 genes. The second study analyzed 2226 NOA patients and 4576 controls in the same population and reported significant associations with SNPs mapping to two regions: HLA-DRA and C6orf10/BTNL2 (Zhao et al. 2012). Despite meeting requirements for genome-wide significant results, no overlapping SNPs were observed between these two large studies. Finally, in the same year Kosova et al. (2012) analyzed 269 Hutterite men and 123 men from Chicago with diverse ethnic background, and described nine SNPs associated with reduced fertility or impaired sperm parameters, but in this case also no SNPs overlapping with the previous three GWAS were reported (Table 2).

Subsequently, SNPs reported as significantly associated or with borderline P values in the above GWAS were analyzed in independent study populations with variable success (Table 3). Findings on the majority of candidate SNPs were not confirmed by the replication studies, and the few SNPs that show association either confer a moderate risk for impaired sperm production or loose significance after Bonferroni correction (for instance, OR2W3, BRDT). Interestingly, the SNP reported in SIRPA/G (rs6080550) with borderline significance in one of the GWAS (Hu et al. 2012) was not confirmed in the follow-up studies, but following re-sequencing of the SIRPA gene, another SNP (rs3197744) was identified as a significant susceptibility factor for oligozoospermia with OR=4.62 (95% CI=1.58–13.4 P=0.005) (Xu et al. 2013) Similarly, the re-sequencing of SIRPG also provided an interesting candidate SNP (rs1048055) with similarly high OR for NOA (OR=3.93, 95% CI=1.59–9.70 P=3.00×10−3) (Lu et al. 2014). Both genes are members of the signal-regulatory-protein (SIRP) family and belong to the immunoglobulin superfamily, and when they bind to CD47 can induce cell apoptosis (Brooke et al. 2004). According to the above data, SIRPA/G can be considered as promising candidate genes for spermatogenic impairment and furtherer investigations.

The HLA-DRA gene-related SNPs turned out to be the most promising, since highly significant association with NOA was found in the GWAS of Zhao et al. (2012) and in four case–control studies in Chinese and Japanese populations (Tsujimura et al. 2002, Jinam et al. 2013, Hu et al. 2014, Tu et al. 2014). HLA-DRA gene is a member of class II genes and encodes the alpha chain of HLA-DR and heterodimerizes with β chains (HLA-DRBs) and plays an important role in the immune system by presenting peptides on the cell surface of antigen-presenting cells. Three variants have been described with significant association with male infertility in Japanese and Chinese populations (Zhao et al. 2012, Jinam et al. 2013, Hu et al. 2014, Tu et al. 2014): rs3129878, rs7194 and rs7192. The variant rs7194 is in linkage disequilibrium with rs7192 and is located on 3′UTR. It was predicted to map to the has-miR-6507-3p binding site and may play an important role during transcription by influencing HLA-DRA expression level through microRNA-mediated post-transcriptional regulation (Lin et al. 2015). As for rs7192, it is a missense variant (L242V) located in exon 4, which encodes part of the DRA α-chain cytoplasmic domain (Neefjes et al. 2011). This SNP might alter interactions with β-chain or ubiquitin E3 ligases, which control the cell-surface expression of class II MHC proteins (Gueant et al. 2015). Finally, rs3129878 maps to intron 1 and its putative effect is not yet clarified. These polymorphisms have been already described as susceptibility factors for a number of autoimmune diseases, therefore it has been hypothesized that they might mediate the response to testicular micro-environmental antigens and therefore may elicit autoimmune inflammatory responses leading to azoospermia (Hu et al. 2012). It would be interesting to study this polymorphism also in Caucasians and in subgroups of patients with previous history of urogenital inflammation, especially orchiepididymitis.

Rare variants: gene re-sequencing studies

Besides the polymorphisms described above, many re-sequencing studies of candidate spermatogenesis genes have been also published. Although many genes are known to be essential for gametogenesis, there are surprisingly few monogenic mutations that have been conclusively demonstrated to cause human spermatogenic failure. The majority of mutations identified are in heterozygosis and therefore the demonstration of a cause-effect relationship remains difficult. In addition, functional studies are lacking in a large majority of the cases. Some of the most promising mutations, for which also functional studies were performed, have been identified in the following genes: i) HSF2 (Mou et al. 2013) and SOHLH1 (Choi et al. 2010) reported in NOA men; ii) NANOS1 (Kusz-Zamelczyk et al. 2013) and NR5A1 (Bashamboo et al. 2010) reported in NOA and oligozoospermic patients; iii) Yatsenko et al. 2006), GALNTL5 (Takasaki et al. 2014) and SEPT12 (Kuo et al. 2012) identified in oligo or OAT men. All the above genes are autosomal and the reported mutations are in heterozygosis. Whether these mutations are fully responsible for the given phenotypes (dominant effect) or are acting in synergy with other yet unidentified heterozygous mutations in genes with similar function (oligogenic model) remains to be defined.

Thanks to the diffusion of NGS platforms, testing for a large panel of candidate genes in large group of patients and controls has now became an affordable approach. The first NGS-based, candidate gene panel study has been recently performed in a Chinese case–control setting including 757 NOA patients and 709 fertile males (Li et al. 2015), Using the HiSDefault 2000 platform, they sequenced a total of 650 infertility-related genes and described a significant excess of rare, non-silent variants in genes that are key epigenetic regulators during spermatogenesis such as BRWD1, DNMT1, DNMT3B, RNf17, UBR2, USP1 and USP26. The authors do not provide detailed information about the exact genotype of the variants, but apparently ‘most of the non-silent variants in these genes in the sporadic NOA patients were heterozygous.’ As USP26 is located on the X chromosome, the reported variants are hemizygous. Given that these genes are involved in similar biological function, the hypothesis about a synergic action of heterozygous mutations is plausible. However, functional analyses are still needed in order to support this hypothesis,

NGS has been recently used with success also for studies of familial cases of azoo/oligozoospermia from Turkey. A novel homozygous mutation in the NPAS2 gene was reported in three brothers from a consanguineous family, showing variable semen phenotypes ranging from azoospermia to oligozoospermia (Ramasamy et al. 2015). Another publication focused on two families: in one case, the most plausible cause for impaired spermatogenesis was a homozygous truncating mutation in TAF4B; in the other case, two azoospermic brothers were homozygous for a mutation in the ZMYND15 gene (Ayhan et al. 2014). All these genes are expressed in the testis and are plausible candidates for the observed phenotypes. However, given that the heterozygous carriers of the families are not affected, mutation screening in sporadic NOA patients has limited, if any, diagnostic relevance.

On the contrary, sex chromosomes represent an optimal target in sporadic cases since mutations are in hemizygosis with a potential direct effect on protein function without a compensating effect from a normal allele. Stouffs & Lissens (2012) have reviewed the literature concerning X-linked gene mutations in eight genes. With the exception of the AR gene, no other causative mutations/polymorphisms have been described with clinical relevance. Novel data on X chromosome-linked genes derives from recent array-CGH studies (see paragraph below) and the most interesting findings concern genes belonging to the cancer testis antigen (CTA) family (Krausz & Giachini 2012) and to a meiosis genes, TEX11 (Yatsenko et al. 2015) (Fig. 1B).

Figure 1

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Figure 1

Schematic representation of sex chromosome-linked CNVs with clinical relevance. (A) Y chromosome CNVs: the picture illustrates complete AZF microdeletions, a direct cause of impaired spermatogenesis and the gr/gr deletion, an ascertained risk factor for spermatogenic impairment. In the lower table, AZF microdeletions and gr/gr deletion frequencies in patients and controls are reported. Azoo: azoospermic; OAT: oligoasthenoteratozoospermic. * mean frequencies of the gr/gr deletion are relative to the Italian and Spanish populations. (B) X chromosome CNVs: DUP1a (Chianese et al. 2014), c.652del237bp in TEX11 (Yatsensko et al. 2015) and CNV67 (Lo Giacco et al. 2014a) are three novel variants with potential clinical implication given their specific association with impaired spermatogenic phenotypes. In the lower table, CNVs type and frequencies in patients and controls are reported. Figure is not in scale.

Citation: REPRODUCTION 150, 5; 10.1530/REP-15-0261

As far as the Y chromosome-linked genes are concerned, studies are limited to deletion analysis rather than intragenic mutation screening, and the only relevant finding concerns the USP9Y gene in the AZFa region (Tyler-Smith & Krausz 2009) Deletions affecting this gene have been associated with a variable semen phenotype from azoospermia to normozoospermia, indicating that the gene is more likely a fine tuner than an essential factors for spermatogenesis.

CNVs and male infertility

CNVs are a class of structural variation that may involve complex gains or losses of homologous sequences at multiple sites in the genome. The first genome-wide map of CNVs existing in the human genome showed that these variations cover ∼360 Mb, i.e., 12% of the human genome and represent the primary source of inter-individual variability between genomes (Redon et al. 2006). Notwithstanding, the gain or loss of DNA sequence can also produce a spectrum of functional effects and human disease phenotypes, by both disrupting gene-coding sequences and affecting region void of genes but involving regulatory elements with an indirect effect on gene transcription. Although the functional consequences of a CNV might be difficult to predict, many CNVs do generate alleles with a clear-cut impact on health and have been associated with a growing number of common complex diseases (Riggs et al. 2014). As infertility is indeed a complex disease, it has been hypothesized that certain CNVs may cause defective recombination (especially those mapping to PAR), leading to meiotic failure and the loss of germ cells, or might affect the activity of individual genes important for spermatogenesis. To date, the only CNVs proved to be in a clear-cut cause-effect relationship with spermatogenic impairment are the AZF microdeletions on the Y chromosome (Vogt et al. 1996, Krausz et al. 2014). Furthermore, the relationship between CNVs and male infertility was also investigated on a larger scale by performing array-CGH on the whole genome (Tüttelmann et al. 2011, Stouffs et al. 2012, Lopes et al. 2013) or at high resolution on the X chromosome (Krausz et al. 2012). The three studies that compared the CNV load between patients and controls all converged on a significantly higher burden of CNVs in men with spermatogenic disturbances (Tüttelmann et al. 2011, Krausz et al. 2012, Lopes et al. 2013). In our study, both the mean number of CNVs/person (mainly dependent on an over-representation of losses) and the mean size/person were significantly increased in the patient group (Krausz et al. 2012). In addition, a significantly lower sperm concentration and total sperm count was found in patients with >1 CNV compared to those with ≤1 CNV. This excess of X-linked CNVs and DNA loss in patients with reduced sperm count and the significant association between CNV number and sperm count in the infertile group support the existence of a potential link between the observed CNV burden and spermatogenic failure. These conclusions are supported also at the whole genome level, but the CNV burden is especially pronounced on the sex chromosomes (Tüttelmann et al. 2011, Lopes et al. 2013). More specifically, Tüttelmann et al. (2011) reported a significant over-representation of sex-chromosomal CNVs in azoospermic men with Sertoli-cell only (SCO) histology, whereas Lopes et al. (2013) in azoo/oligozoospermic men.

Sex chromosomes

Sex chromosomes clearly play an important role in spermatogenesis since they are enriched with genes involved in the development and differentiation of gonads and gametogenesis (Skaletsky et al. 2003, Mueller et al. 2008, 2013). Given that with the exception of the PAR genes, men are hemizygous for most of the genes located on this chromosome, any de novo mutation/CNV might have an immediate impact, since no compensation is provided by another normal allele. Moreover, both chromosomes have accumulated a relevant number of segmental duplications (also called amplicons), which constitute a favorable substrate for CNV formation.

The Y chromosome

The Y chromosome: as already mentioned, Y chromosome microdeletions occurring on the AZF region are the first and thus far the only example of CNVs with clinical significance (Krausz et al. 2014). While the complete AZF deletions have been introduced as a routine genetic test for patients with severe OAT and NOA, the role of partial AZFc deletions, i.e., gr/gr deletion, b1/b3, b2/b3 (Repping et al. 2003, 2004) has been the object of long-lasting debates (Fig. 1A). Four meta-analyses are available on the gr/gr deletion and all reach significant odds ratios, reporting on average two- to 2.5-fold increased risks of reduced sperm output/infertility (Tüttelmann et al. 2007, Visser et al. 2009, Navarro-Costa et al. 2010, Stouffs et al. 2011). In a more recent survey on AZFc deletions in a sample of 20 884 men, Rozen et al. (2012) found the gr/gr deletion to be the most common among partial AZFc deletions (2.4% or 1/41 men), as well as that it doubles the risk for impaired spermatogenesis. These data altogether thus confirm the gr/gr deletion as an established significant genetic risk factor for impaired sperm production. The entity of the risk associated with this genetic anomaly varies between populations, reaching the highest OR in Italians, which have a 7.9-fold increased risk for spermatogenic impairment (OR=7.9, 95% CI 1.8–33.8) (Ferlin et al. 2005, Giachini et al. 2005, 2008). The existence of Y chromosomal haplogroups that constitutively carry the gr/gr deletion, such as the Db2 branch common in Japan and the Q1 haplogroup common in China, indicates that the Y background may modulate the penetrance of this CNV in Asia (Repping et al. 2006, Zhang et al. 2007). Interestingly, phenotypic variation within European carriers of the Y-chromosomal gr/gr deletion is independent of the Y-chromosomal background (Krausz et al. 2009).

Though Y-chromosome microdeletions are directly associated only with spermatogenic failure, concerns have been raised about the potential risk for carriers undergoing assisted reproductive technology to father children affected not only by impaired spermatogenesis but also other conditions such as Turner's syndrome (45,X) and other phenotypic anomalies associated with sex chromosome mosaicism (e.g., ambiguous genitalia) (Patsalis et al. 2002, Krausz et al. 2014). Furthermore, a recent study (Jorgez et al. 2011) reported that 5.4% of men with AZF deletions and a normal karyotype also carried SHOX haploinsufficiency. Indeed, this information raised the question about the importance of screening for SHOX-linked CNVs in men carrying Y-chromosome microdeletions. Our group performed a large multicenter study in order to evaluate whether such an alarming hypothesis was actually true (Chianese et al. 2013). No association was found between Y-chromosome microdeletions and SHOX haploinsufficiency, implying that deletion carriers have no augmented risk of SHOX-related pathologies (short stature and skeletal anomalies).

The question whether increased gene dosage of the AZFc region may also affect fertility originates from the observation of a limited variation in the copy number of AZFc-linked genes, which strongly indicates a natural selection for the conservation of an ‘optimal’ copy number by removing exceptionally high or low copy number variants from the population (Repping et al. 2006). The DAZ gene in the AZFc region is a clear example: about 90% of men carry four DAZ copies, which suggests that this is the optimal number required for normal spermatogenesis and that both a reduction and an increase of AZFc gene dosage may have a negative effect. This observation encouraged initially two groups to investigate the clinical consequences of partial AZFc duplications, reaching different conclusions: an association between increased AZFc gene dosage and male infertility was observed in the Han Chinese study (Lin et al. 2007), whereas no association could be detected in the Italian study population (Giachini et al. 2008). Later on, the effect of AZFc duplications on spermatogenesis was further investigated and again different results were obtained. Ye et al. (2013) found a significantly higher frequency of partial duplications in the infertile patients (4.0%) compared to controls (0.7%) in the Chinese-Yi population. Contrastingly, in the analysis by Lo Giacco et al. (2014a), performed on a study population including prevalently Spanish subjects, AZFc duplications were found at comparable frequencies in patients (4.9%) and controls (3.5%). Seemingly, this discordance reflects mere ethnic differences; therefore, if increased AZFc gene content does play a role in spermatogenic impairment, the effect is probably modulated by population-specific factors.

The X chromosome

The first X chromosome studies were based on the candidate gene approach, and a total of seven X-linked candidate genes have been studied so far (AR, AKAP, FATE, NXF2, TAF7L, SOX3, USP26). With the exception of the AR gene, no clear-cut causative mutations have been reported and SNPs linked to some of these genes have been the objects of discordant results (Table 1). With the shift of discovery research to high-throughput approaches, researchers were encouraged to apply such technologies to investigate X chromosome-linked CNVs and their role in spermatogenic failure. To date, four groups have employed comparative genomic hybridization (CGH) arrays (Tüttelmann et al. 2011, Krausz et al. 2012, Stouffs et al. 2012, Lopes et al. 2013) and three provide information about X-linked CNVs with potential clinical relevance in the etiology of male infertility (Tüttelmann et al. 2011, Krausz et al. 2012, Lopes et al. 2013) (Fig. 1B).

The analysis performed by array-CGH employing a high-resolution (probe distance of 2–4 Kb) X chromosome-specific platform (Krausz et al. 2012) allowed the identification of a consistent number of CNVs on the X chromosome, the majority of which (75.3%) were novel. From a clinical standpoint, of particular interest are patient-enriched (significantly more frequent in patients) and patient-specific (not found in controls) CNVs, since genes and regulatory elements within or nearby these regions presumably have a higher probability of being implicated in spermatogenic failure. Although there are some partially overlapping findings regarding the X chromosome-linked CNVs between the three studies (Tüttelmann et al. 2011, Krausz et al. 2012, Lopes et al. 2013), differences in the resolution of the arrays may explain the lack of complete overlaps. By performing a comparison between the raw data of the three studies we observed a few interesting overlapping CNVs. Three patient-specific CNVs – DUP1a, DUP55 and DUP60 – detected in the study by Krausz et al. (2012) were also found by Tüttelmann et al. (2011) in men affected by SCOS. The comparison with data by Lopes et al. (2013) also shows an overlap of a recurrent deletion detected in their study at a significantly higher frequency in patients compared to controls and two patient-specific CNVs, CNV30 (gain) and CNV31 (loss), identified in the Krausz’ study. When comparing patient-specific CNVs detected in the study by Tüttelmann et al. (2011), the loss nssv1496532 overlaps with CNV69, which was found significantly more frequent in patients than controls in the Krausz’ study. One gain on Xq22.2 (Lopes et al. 2013) overlapped with the private duplication nssv1499049 found in an oligozoospermic man in Tüttelmann's study. It is worth noting that this duplication intersects a number of genes with specific or exclusive expression in the testis (H2BFWT, H2BFXP and H2BFM). No CNVs were found to be common to all three studies. In the light of these comparisons, DUP1a, CNV69 and the nssv1499049 are promising variants, since their potential involvement in spermatogenic impairment was reported by more than one study.

In fact, the two variants DUP1a and CNV69 were objects of large follow-up studies, together with other recurrent deletions, CNV67 and CNV64 (Chianese et al. 2014, Lo Giacco et al. 2014b). The first study analyzed three recurrent deletions (frequency >1%) in a large case–control setting (n=1255) for their exclusive (CNV67) and prevalent (CNV64 and CNV69) presence in patients. For instance, deletion carriers displayed a higher probability of having impaired spermatogenesis (OR=1.9 and 2.2 for CNV64 and CNV69 respectively) as well as sperm concentration and total motile sperm number was lower in carriers compared to non-carriers The most interesting deletion was CNV67 because it was exclusively found in patients with a frequency of 1.1% (P<0.01) and is likely to involve the MAGE9A gene – a CTA family member – and/or its regulatory elements (Lo Giacco et al. 2014b). Similarly, a follow-up study was performed on five selected gains (DUP1A, DUP5, DUP20, DUP26 and DUP40), which include, or are in close proximity to, genes with testis-specific expression and potential implication in spermatogenesis (Chianese et al. 2014). While four of the five CNVs (DUP5, DUP20, DUP26 and DUP40) did not individually reach statistical significance, they remained patient-specific. DUP1A, instead, was found exclusively and at a significantly higher frequency in patients. This gain fully duplicates a long non-coding RNA (LINC00685) that potentially acts as a negative regulator of a gene with potential role in spermatogenesis, PPP2R3B; according to our hypothesis, the mechanism by which DUP1A could lead to spermatogenic failure is a misbalanced ratio of the PPP2R3B and its antisense, causing a decrease in PPP2R3B transcription in the developing germ cells (Chianese et al. 2014). Our data together with the identification of two SCOS patients with a duplication disrupting the PPP2R3B gene (Tüttelmann et al. 2011) indicate that CNVs mapping into this region and affecting either PPP2R3B or the long non-coding RNA (LINC00685) are good mutational targets for future case–control studies.

Lastly, a recent study proved the implication of the TEX11 gene in meiotic arrest and azoospermia (Yatsenko et al. 2015). The study population included a total of 289 patients with different testis histology (63 with SCOS, 33 with meiotic arrest and 193 with mixed testicular atrophy) and 384 normozoospermic controls. With the use of an X-chromosome high-resolution GCH microarray, they firstly analyzed 15 azoospermic men and found that a patient with mixed atrophy carried a 91-KB deletion (c.652del237bp) encompassing exons 10, 11 and 12 of TEX11. Further Sanger sequencing in the rest of the patients allowed detecting that another man with meiotic arrest carried the same deletion c.652del237bp, which was confirmed by array-CGH validation; moreover, they found five patients with either meiotic arrest or mixed testicular atrophy carrying missense mutations in TEX11. None of the controls carried any of these variants. Finally, the finding of TEX11 mutations in 2.4% (n=7/289) of patients, of which 15% (n=5/33) suffered from meiotic arrest and 1% (n=2/193) had a mixed testicular atrophy, supports the importance of this gene for normal spermatogenesis.


Whole-genome approaches allowed providing data also on the potential role of autosome-linked CNVs in relation to different semen phenotypes (Tüttelmann et al. 2011, Stouffs et al. 2012, Lopes et al. 2013). The first study reported eight autosomal rearrangements (involving chromosomes 1, 2, 3, 5, 12, 15, 16, 17) potentially linked to fertility problems, as they were not detected in normozoospermic controls (Stouffs et al. 2012). The second study reported recurrent and patient-specific autosomal CNVs potentially associated with oligozoospermia (n=11) and with SCOS (n=4), also reporting a list of genes intersecting the CNVs and with potential involvement in the spermatogenic phenotype. Finally, after assaying genome-wide SNPs and CNVs, the third study estimated that rare autosomal deletions multiplicatively change a man's risk of disease by 10% (OR 1.10 (1.04–1.16), P<2×10−3). The same authors observed five deletions (ranging in size from 54 kb to over 2 Mb) of the autosomal DMRT1 gene in four cases of azoospermia and one in normozoospermia. Despite the normozoospermic deletion carrier, statistical analysis based on the comparison of all patients versus 7000 controls lead to a significant association with impaired sperm production. Given the low frequency of this mutation and the wide range of associated phenotype, it remains difficult to include the testing for DMRT1-linked CNVs in the routine diagnostic workup.

The comparison between the three studies shows some overlapping findings. When comparing the CNVs detected by Stouffs et al. (2012) with the raw data deposited in dbVar by Tüttelmann et al. (2011), five overlapping loci can be observed on chromosomes 1, 5, 15, 16 and 17, but only those related to chromosome 1 and 16 results are patient-specific in both studies. The first locus on chromosome 1 shares a 46 kb-span overlap with the gain nssv1495850 reported in an oligozoospermic man in Tüttelmann's study. The other locus on chromosome 16 overlaps with both gains and losses from Tüttelmann's study; interestingly, gains are found in both patients and controls, whereas the reciprocal losses were exclusively detected in OAT patients. When comparing the Lopes’ and the Tüttelmann's study, one overlap is reported on chromosome 8: at this locus, Tuttelmann et al. identified a deletion in an azoospermic man and another with a duplication, intersecting the PLEC1 and MIR661 genes, whereas Lopes et al. identified a duplication in an oligozoospermic man affecting the same genes. No CNVs were observed to be common to all three studies.

Summary and future directions

Male infertility is a multifactorial complex disease with highly heterogeneous phenotypic representation. The wide range of quantitative and qualitative impairments can be caused by several acquired and congenital factors, including genetic/epigenetic anomalies. Despite a 10-year effort, research was largely unsuccessful in identifying recurrent genetic factors with potential clinical application. The armamentarium of diagnostic tests has been implemented only by the screening for Y chromosome-linked gr/gr deletion in those populations for which robust and consistent data with risk estimate are available. Much expectation was given to genome-wide SNP arrays, based on the analysis of common variants, but no overlapping SNPs have been identified between different studies. Meta-analyses have been able to demonstrate significant association only for a few SNPs, conferring generally weak predisposition to infertility. According to a few observations, common SNPs with significant but low effect size may eventually lead to impaired spermatogenic efficiency if they are present contemporarily in the same individual (Aston et al. 2010, Kosova et al. 2012). On the other hand, it is clearly demonstrated by both SNP and array-CGH, that there is a rare variant burden in men with impaired spermatogenesis, which is especially relevant concerning CNVs. Whether this phenomenon is an expression of a more generalized genomic instability is still an open question. Epidemiological observations indicating lower life expectancy and higher morbidity in infertile men (Jensen et al. 2009, Salonia et al. 2009, Eisenberg et al. 2014) are suggestive for such a potential relationship.

It has been predicted that more than 2000 genes (housekeeping and specific germ cell genes) are involved in spermatogenesis (Hochstenbach & Hackstein, 2000) and mutation in these genes may act directly or through gene-environmental interaction. In the era of NGS we expect to expand our diagnostic skills, since mutations in several hundred of genes can potentially lead to infertility and each of them is likely responsible for only a small fraction of cases. Exome analysis is predicted to be successful especially for descendants of consanguineous families and familial cases of infertility. Concerning sporadic oligo/azoospermia, the situation is more complex and, since the infertile trait undergoes negative selection, at least two scenarios can be predicted. On one hand, there is a possibility that rare or de novo large-effect mutations are involved in these pathological conditions; in this regard, the X chromosome represents one of the most exciting future targets for both its enrichment in genes involved in spermatogenesis and its hemizygous state in males, which implies a direct effect of a damaging mutation. On the other hand, an alternative pathogenic mechanism can be related to a synergistic effect of multiple heterozygous mutations in genes involved in the same biological pathway. In this regard, system biology, which allows unrevealing possible gene interactions and common biological pathways, will provide an informative tool for NGS data interpretation. Although these novel approaches will certainly help discover ‘hidden’ genetic factors, a more comprehensive picture of the etiopathogenesis of idiopathic male infertility will only be achieved by a parallel investigation of the complex world of gene environmental interaction and epigenetics.

Supplementary data

This is linked to the online version of the paper at

Declaration of interest

The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of this review.


This work was supported by the following funding agencies: European Commission, Reproductive Biology Early Research Training (REPROTRAIN, Project Number: 289880) and the Spanish Ministry of Health (FIS grant PI14/01250).


The authors thank Gianni Forti (University of Florence) and Esperança Marti Salas (Fundació Puigvert) for their continuous support to research in genetics of male infertility. We also thank the following funding agencies: European Commission, Reproductive Biology Early Research Training (REPROTRAIN, Project Number: 289880), IBSA Foundation for Scientific Research to CC and the Spanish Ministry of Health (FIS grant PI14/01250).


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    Schematic representation of sex chromosome-linked CNVs with clinical relevance. (A) Y chromosome CNVs: the picture illustrates complete AZF microdeletions, a direct cause of impaired spermatogenesis and the gr/gr deletion, an ascertained risk factor for spermatogenic impairment. In the lower table, AZF microdeletions and gr/gr deletion frequencies in patients and controls are reported. Azoo: azoospermic; OAT: oligoasthenoteratozoospermic. * mean frequencies of the gr/gr deletion are relative to the Italian and Spanish populations. (B) X chromosome CNVs: DUP1a (Chianese et al. 2014), c.652del237bp in TEX11 (Yatsensko et al. 2015) and CNV67 (Lo Giacco et al. 2014a) are three novel variants with potential clinical implication given their specific association with impaired spermatogenic phenotypes. In the lower table, CNVs type and frequencies in patients and controls are reported. Figure is not in scale.

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