There is little information on the accessibility of the seminiferous tubule to serum proteins and especially to the glycoprotein hormones or to immunoglobulins. The secretions of ram seminiferous tubules may be collected from the rete testis by inserting a cannula via the vasa efferentia (Voglmayr, Scott, Setchell & Waites, 1967), and this paper reports the results of an analysis of these secretions for protein and immunoglobulin.
The Biuret reaction was used to determine the total protein concentration of rete testis fluid collected at different times of the year from rams showing wide seasonal variation in spermatogenesis. The values for rams showing active spermatogenesis were not different from those with poor spermatogenesis (Table 1). The protein content was much lower than that of testicular lymph,
Present address: Ian Clunies Ross Animal Research Laboratory, C.S.I.R.O.
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