IMMUNOCHEMICAL STUDIES ON HUMAN SEMINAL PLASMA

in Reproduction
Authors:
SIDNEY SHULMAN
Search for other papers by SIDNEY SHULMAN in
Current site
Google Scholar
PubMed Close
and
PAUL BRONSON
Search for other papers by PAUL BRONSON in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1530/jrf.0.0180481
Volume/Issue:
Volume 18: Issue 3
Page Range:
481–491
Article Type:
Research Article
Online Publication Date:
Apr 1969
Free access

Sign up for journal news

Summary.

It was found that human seminal plasma contained a large number of antigenic components as tested with selected antiserum bleedings from rabbits that had been intensively immunized with pooled seminal plasma. Gel diffusion experiments revealed a total of eight to eleven distinctive components, although several experiments had to be considered jointly to achieve unambiguous resolution. These experiments included variations in the concentration of the seminal plasma and the spacings between the wells.

Immuno-electrophoresis gave a clearer resolution. A characteristic pattern of twelve components was seen, consisting of four major mobility groups of antigens with high diffusion rates and two other mobility groups with low diffusion rates. These were termed A, B1, B2, C1, C2, C3, D1, D2, E1, E2, F1 and F2. It was further shown that serum albumin was one of the components in seminal plasma (component A).

It was also found that the predominant electrophoretic component could be isolated by starch block electrophoresis, and that this corresponded to component C2 of the immuno-electrophoretic pattern.

Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 102 17 1
PDF Downloads 93 29 2

 

  • Collapse
  • Expand
Print ISSN:
1470-1626
Online ISSN:
1741-7899