The pituitary contents of fsh (hcg augmentation method of Steelman & Pohley) and lh (ovarian ascorbic acid depletion method of Parlow) were determined at 0, 2, 9, 13 and 25 weeks and at 0, 2 and 13 weeks, respectively, after bilateral spaying. The fsh concentration (μg/mg) increased up to 9 weeks with no changes thereafter, but the fsh content (μg/gland) and also lh levels increased up to 13 weeks.
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