Mouse ovaries were incubated with fsh then removed to fresh medium lacking hormone. At various times after removal, [14C]thymidine was added to the dishes. After 24-hr further incubation, the tissue was prepared for counting. Parallel cultures, never exposed to fsh, received thymidine and were counted simultaneously. The difference in counts between paired fsh-treated and untreated cultures indicated the residual fsh effect. The differences were significant up to 9 hr after removal from fsh, but not thereafter. The results suggest that a constant supply of fsh is needed for the continued growth of early follicles.
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