Four treatment methods for the preparation of deep-frozen bull semen were tested for fertility in a factorial design involving the artificial insemination of 1728 first-service cows with the following results :
of non-returns to service at 3 months, with equal treatment groups of 432 cows. The improvement in fertility after equilibration in the presence of fructose was highly significant (P < 0·01), but not in its absence, when fertility was depressed even in equilibrated semen.
There was a higher revival rate of spermatozoa after the longer period of equilibration and the use of fructose in the diluent significantly improved the percentage of motile spermatozoa after thawing.
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