The volumes of Fallopian tube secretion which pass into the peritoneal cavity (ampullar flow) and uterus (isthmic flow) were recorded at 12-hr intervals in ovariectomized ewes, and after injection of 30 μg, 90 μg or 500 μg oestradiol benzoate (ODB). Ovariectomized ewes were also treated sequentially with progesterone, oestrogen, and, in some animals, a further series of progesterone injections in an attempt to achieve the pattern of directional flow of tubal fluid found in the intact, cyclic ewe.
A decline in total daily secretion and fluid flow followed ovariectomy and was counteracted by the administration of ODB. Both ampullar and isthmic flows were markedly increased at all dose levels of ODB until the flows were comparable with, or exceeded, those which occurred during oestrus in the intact ewe. But the sequence of maximum ampullar flow and maximum isthmic flow was reversed by ODB, the difference being further accentuated by each increase in dose level. As isthmic flow was stimulated at all dose levels of ODB, an effective restriction of the tubo-uterine junction and/or isthmus was not apparent. Progesterone in combination with ODB did not effectively restrict the isthmic flow relative to the ampullar flow.
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