APPLICATION OF EOSIN FOR DIFFERENTIAL STAINING OF BUFFALO SPERMATOZOA

in Reproduction
Buffalo semen has received relatively little attention owing to the fact that buffaloes are mainly found in recently developing countries, where artificial insemination is not widely applied. The methods routinely followed in artificial insemination may require to be modified so as to suit the nature and peculiarities of this species. In the process of semen evaluation, the motility test has a special importance, as fertilization is expected only when there is a certain percentage of active, progressively motile spermatozoa. Differential staining can be used for estimating the percentage of live spermatozoa. Staining with opal blue—eosin mixture has been applied to a certain extent in semen evaluation. Lasley & Bogart (1943) employed this mixture for beef cattle and reported a correlation of +0·41 between the percentage of live spermatozoa and motility, and +0·83 between fertility and the percentage of live spermatozoa. Emik & Sidwell

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