RELATIONSHIP OF A TRYPSIN-LIKE ENZYME IN RABBIT SPERMATOZOA TO CAPACITATION

in Reproduction

Summary.

The existence of a trypsin-like enzyme (TLE) in acrosomes of epididymal spermatozoa was confirmed and was further demonstrated to be present in acrosomes of ejaculated and capacitated spermatozoa. TLE rapidly removes the zona pellucida of the ovum. Extracts of acrosomes of ejaculated spermatozoa contain an inhibitor that is separated from the TLE by purification of the TLE.

The inhibitor of TLE is also present in seminal plasma. This enzyme— enzyme inhibitor relationship appears analogous to the corona-removing enzyme-decapacitation factor relationship and part of capacitation very likely involves removal of the inhibitor from TLE and decapacitation factor from the corona-removing enzyme. TLE is inhibited by soybean trypsin inhibitor and less effectively by mercaptoethanol.

If the inline PDF is not rendering correctly, you can download the PDF file here.

 

     An official journal of

    Society for Reproduction and Fertility

 

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 127 28 1
PDF Downloads 64 32 3