CHARACTERIZATION AND ISOLATION OF A SPERM-COATING ANTIGEN FROM RABBIT SEMINAL PLASMA WITH CAPACITY TO BLOCK FERTILIZATION

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Authors:
A. G. HUNTER
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and
H. O. NORNES
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DOI:
https://doi.org/10.1530/jrf.0.0200419
Volume/Issue:
Volume 20: Issue 3
Page Range:
419–427
Article Type:
Research Article
Online Publication Date:
Dec 1969
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Summary.

The relationship between the sperm-coating antigens of rabbit seminal plasma and the phenomenon of decapacitation was studied using agar-gel diffusion, immuno-electrophoresis, chromatography on Sephadex G-200, and polyacrylamide vertical gel electrophoresis.

Interpretation of data obtained with these techniques led to the conclusion that a sperm-coating antigen of seminal plasma origin possessed biological activity for blocking fertilization. The sperm-coating antigen was a glycoprotein of approximately 170,000 molecular weight, migrated in an electric field similar to a serum slow β-globulin and was still present in the seminal fluid of vasectomized males. This sperm-coating antigen was absent from the inactive upper supernatant fluid fraction of seminal plasma after 4 hr of ultracentrifugation at 105,000 g and was present in the active ultracentrifugal pellet.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899