THE FIXATION OF CARBON DIOXIDE BY THE EIGHT-CELL MOUSE EMBRYO

in Reproduction
Mouse embryos require the presence of a bicarbonate buffer system for their development in vitro. The energy requirements for oocyte maturation and early zygote development prompted Biggers, Whittingham & Donahue (1967) to suggest that the fixation of carbon dioxide may play a rôle in the energy metabolism of the early embryo. The present study was undertaken in an attempt to demonstrate this reaction in the eight-cell mouse embryo.For each experiment, 400 eight-cell mouse embryos were flushed from the reproductive tracts of superovulated female albino mice. The basic medium was Krebs-Ringer bicarbonate containing 25 mm-sodium dl lactate, 0·25 mm-sodium pyruvate, 0·01 mm-sodium ketoglutarate, 0·01 mm-sodium malate, 0·01 mm-sodium citrate, 1 mg/ml bovine serum albumin, 60 μg/ml penicillin, 50 μg/ml streptomycin. After washing by transfer through

If the inline PDF is not rendering correctly, you can download the PDF file here.

 

     An official journal of

    Society for Reproduction and Fertility

 

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 56 12 0
PDF Downloads 135 13 1